Back to Search Start Over

Initial sequence and comparative analysis of the cat genome

Authors :
Natalia Volfovsky
Jill Pecon-Slattery
James C. Mullikin
Kristina Narfström
Naoya Yuhki
Marilyn Menotti-Raymond
Warren E. Johnson
Alfred L. Roca
Michele Clamp
Urs Giger
Agencourt Sequencing Team
Richa Agarwala
Robert M. Stephens
Agostinho Antunes
Douglas Smith
Glenn Tesler
Kerstin Lindblad-Toh
Stephen J. O'Brien
Jean Chang
Alejandro A. Schäffer
Joan Pontius
Guillaume Bourque
Nisc Comparative Sequencing Program
Sante Gnerre
Beena Neelam
William J. Murphy
Source :
Genome Research
Publication Year :
2007
Publisher :
Cold Spring Harbor Laboratory Press, 2007.

Abstract

The genome sequence (1.9-fold coverage) of an inbred Abyssinian domestic cat was assembled, mapped, and annotated with a comparative approach that involved cross-reference to annotated genome assemblies of six mammals (human, chimpanzee, mouse, rat, dog, and cow). The results resolved chromosomal positions for 663,480 contigs, 20,285 putative feline gene orthologs, and 133,499 conserved sequence blocks (CSBs). Additional annotated features include repetitive elements, endogenous retroviral sequences, nuclear mitochondrial (numt) sequences, micro-RNAs, and evolutionary breakpoints that suggest historic balancing of translocation and inversion incidences in distinct mammalian lineages. Large numbers of single nucleotide polymorphisms (SNPs), deletion insertion polymorphisms (DIPs), and short tandem repeats (STRs), suitable for linkage or association studies were characterized in the context of long stretches of chromosome homozygosity. In spite of the light coverage capturing ∼65% of euchromatin sequence from the cat genome, these comparative insights shed new light on the tempo and mode of gene/genome evolution in mammals, promise several research applications for the cat, and also illustrate that a comparative approach using more deeply covered mammals provides an informative, preliminary annotation of a light (1.9-fold) coverage mammal genome sequence.

Details

Language :
English
Database :
OpenAIRE
Journal :
Genome Research
Accession number :
edsair.doi.dedup.....5a517d70d1b66834cae58e03e9af6916