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Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit
- Source :
- International Journal of Molecular Sciences, RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname, Volume 22, Issue 18, International Journal of Molecular Sciences, Vol 22, Iss 9716, p 9716 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- [EN] Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.<br />This research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO), Spain, grant number AGL2014-53405-C2-1-P and by Conselleria d'Educacio, Investigacio, Cultura i Esport, Spain, grant number Prometeo II 2014/036. Ximo Garcia-Dominguez was supported by a research grant from the Ministry of Economy, Industry and Competitiveness of Spain (BES-2015-072429).
- Subjects :
- Epigenomics
Male
Proteomics
Proteome
medicine.medical_treatment
PRODUCCION ANIMAL
Epigenesis, Genetic
stress
Pregnancy
Biology (General)
Spectroscopy
DNA methylation
Genome
Reproduction
Embryo
General Medicine
steroid biosynthesis
Embryo transfer
Computer Science Applications
Chemistry
BIOLOGIA ANIMAL
Liver
Metabolome
Female
Steroids
Assisted reproductive technologies
Rabbits
Steroid biosynthesis
Reproductive Techniques, Assisted
QH301-705.5
Offspring
Biology
Stress
Catalysis
Article
Inorganic Chemistry
Andrology
medicine
Animals
Humans
Epigenetics
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
assisted reproductive technologies
Organic Chemistry
Lipid metabolism
Embryo Transfer
Embryo, Mammalian
Lipid Metabolism
Vitrification
GENETICA
Steroid hormone
Pregnancy, Animal
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....5a4d53c0b3a84bf95609c4e32e2f1910