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Increased STAT3 phosphorylation on CD27 + B-cells from common variable immunodeficiency disease patients
- Source :
- Clinical Immunology. 161:77-88
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Maturation and differentiation of B-cells are driven by T-cells' help through IL-21/STAT3 axis in GC centers or through extrafollicular pathways, in a T-independent manner. B-cell differentiation is defective in common variable immunodeficiency disease (CVID) patients. We investigated if IL-21/STAT3 axis alterations could influence B-cell fate. We activated purified CVID B-cells with surrogate T-dependent (anti-CD40), T-independent (TLR-9 ligand) stimuli or through B-cell receptor engagement (anti-IgM) with or without IL-21. IL-21 mediated STAT3 activation was greater on CD27(-) than CD27(+) B-cells depending on the stimulus. IL-21 alone induced STAT3 phosphorylation (pSTAT3) only on CD27(-) B-cells and IL-21 induced higher pSTAT3 levels on CD27(-) than CD27(+) B-cells after anti-IgM or anti-CD40 activation. CVID CD27(+) B-cells showed selective STAT3 hyperphosphorylation after activation with anti-IgM or anti-CD40 alone and anti-IgM, anti-CD40 or ODN combined with IL-21. Increased STAT3 activation during immune responses could result in B-cell differentiation defects in CVID.
- Subjects :
- STAT3 Transcription Factor
T-Lymphocytes
Cellular differentiation
Immunology
Receptors, Antigen, B-Cell
Hyperphosphorylation
Lymphocyte Activation
Immune system
medicine
Humans
Immunology and Allergy
CD40 Antigens
Phosphorylation
B-cell activating factor
Receptor
B-Lymphocytes
CD40
biology
Interleukins
Common variable immunodeficiency
Cell Differentiation
hemic and immune systems
medicine.disease
Molecular biology
Antibodies, Anti-Idiotypic
Tumor Necrosis Factor Receptor Superfamily, Member 7
Common Variable Immunodeficiency
biology.protein
Cancer research
Subjects
Details
- ISSN :
- 15216616
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- Clinical Immunology
- Accession number :
- edsair.doi.dedup.....5a486d6e55d86a0754bef5918b41a0cb