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Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis
- Source :
- Molecular plant. 11(7)
- Publication Year :
- 2017
-
Abstract
- Manganese (Mn) is an essential catalytic metal in the Mn-cluster that oxidizes water to produce oxygen during photosynthesis. However, the transport protein(s) responsible for Mn 2+ import into the chloroplast remains unknown. Here, we report the characterization of Arabidopsis CMT1 (Chloroplast Manganese Transporter 1), an evolutionarily conserved protein in the Uncharacterized Protein Family 0016 (UPF0016), that is required for manganese accumulation into the chloroplast. CMT1 is expressed primarily in green tissues, and its encoded product is localized in the inner envelope membrane of the chloroplast. Disruption of CMT1 in the T-DNA insertional mutant cmt1-1 resulted in stunted plant growth, defective thylakoid stacking, and severe reduction of photosystem II complexes and photosynthetic activity. Consistent with reduced oxygen evolution capacity, the mutant chloroplasts contained less manganese than the wild-type ones. In support of its function as a Mn transporter, CMT1 protein supported the growth and enabled Mn 2+ accumulation in the yeast cells of Mn 2+ -uptake deficient mutant ( Δsmf1 ). Taken together, our results indicate that CMT1 functions as an inner envelope Mn transporter responsible for chloroplast Mn 2+ uptake.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Photosystem II
Mutant
Arabidopsis
chemistry.chemical_element
Plant Science
Manganese
Oxygen-evolving complex
Biology
Photosynthesis
01 natural sciences
Thylakoids
03 medical and health sciences
Gene Expression Regulation, Plant
Homeostasis
DNA (Cytosine-5-)-Methyltransferases
Molecular Biology
Arabidopsis Proteins
food and beverages
Photosystem II Protein Complex
Biological Transport
Transport protein
Cell biology
Chloroplast
Plant Leaves
030104 developmental biology
chemistry
Thylakoid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17529867
- Volume :
- 11
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Molecular plant
- Accession number :
- edsair.doi.dedup.....5a41efa02eda78ab75995bd4fbf77847