Additional file 1 of Proteome network analysis of skeletal muscle in lignan-enriched nutmeg extract-fed aged mice

Additional file 1. Figure 1 Isolation and enrichment of ethanol-extracted LNX. (A) UV chromatogram of ethanol-extracted LNX and yields of main lignan compounds from LNX. Mean±St Dev. Standard deviation was calculated by triplicate experiments. (B) Chemical structures of main lignan compounds from LNX. Analysis condition: equipment, Agilent 1200 series HPLC system; mobile phase, acetonitrile: 0.1% formic acid (4:6); stationary phase, Waters C18 column (5 μm pore size, 25 cm in length); detection wavelength, 234 nm; flow rate, 1.5 ml/min. Figure 2 Dissection and microscopic images of skeletal muscles of LNX-fed mice. (A) Images of skeletal muscles. Left and right panel on each picture indicates fore and hind limb, respectively. Scale bar is 1 cm. (B) H&E staining of cross sections from gastrocnemius muscle and tibialis anterior. Abbreviations: mfb, microfibrils; mf, microfiber; pm, perimysium. Arrows indicate nuclei staining. Scale bar = 20 μm. Figure 3 Anatomical changes of livers in (A) young, (B) LNX (100 mpk) and (C) creatine (120 mpk)-fed old mice. Scale bar = 1 cm. Figure 4 Box plots of all proteome quantitates from four groups: young, old, LNX-fed old mice. Doses of LNX applied to old mice were 10, 30, and 100 mpk. Proteomic sample source was soleus muscle from each group. Figure 5 Heat map analysis of mouse soleus muscle proteomes. Significantly altered 16 proteins in LNX-fed old vs. old mice soleus muscles. Red and blue color intensity means the degree of up- and down-regulation, respectively.