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Galectin-9 in Allergic Airway Inflammation and Hyper-Responsiveness in Mice
- Source :
- International Archives of Allergy and Immunology. 151:308-317
- Publication Year :
- 2009
- Publisher :
- S. Karger AG, 2009.
-
Abstract
- Background: Galectin-9 (Gal-9) is a member of the growing family of β-galactoside-binding lectins. Gal-9 is an eosinophil chemoattractant and inducer of Th1 cell apoptosis. These effects suggest its potential role in the pathogenesis of asthma. Our aim was to study the expression of Gal-9 in an ovalbumin (OVA)-induced mouse model of allergic asthma. Methods: To investigate the significance of Gal-9 in allergic inflammation and airway hyperresponsiveness (AHR), a group of BALB/c mice was sensitized and challenged with OVA (GOVA). Another group of animals was allergized with OVA and also treated with dexamethasone (DEX) (GOVA+DEX). The control group (GPBS) received phosphate-buffered saline instead of OVA as placebo. Airway reactivity to intravenous methacholine was assessed. Results: The percentage of Gal-9-positive cells and their intracellular Gal-9 content and Th1/Th2 cytokine levels in the bronchoalveolar lavage (BAL) were determined by flow cytometry. Gal-9 mRNA expression and protein level were measured in the lung tissue by real-time RT-PCR and Western blot. In GOVA mice, airway inflammation and mucus hypersecretion developed. DEX treatment inhibited the main features of experimental asthma. The number of Gal-9-positive lymphocytes, eosinophil and neutrophil granulocytes and the levels of Th2 cytokines were higher in the BAL of GOVA compared to GPBS or GOVA+DEX mice. Moreover, Gal-9 protein level was elevated in the lungs of GOVA mice. Conclusions: These results suggest that Gal-9 plays a role as a mediator contributing to the development of allergic airway inflammation. Gal-9 may serve as a recruiter of eosinophil granulocytes and promoter of Th2 dominance.
- Subjects :
- Allergy
Ovalbumin
Galectins
Immunology
Anti-Inflammatory Agents
Inflammation
Granulocyte
Dexamethasone
Allergic inflammation
Mice
Cell Movement
Animals
Immunology and Allergy
Medicine
Inducer
Methacholine Chloride
Galectin
Mice, Inbred BALB C
business.industry
General Medicine
Allergens
Eosinophil
medicine.disease
Asthma
Respiratory Function Tests
Disease Models, Animal
medicine.anatomical_structure
Microscopy, Fluorescence
Apoptosis
Cytokines
Female
medicine.symptom
business
Bronchoalveolar Lavage Fluid
Granulocytes
Subjects
Details
- ISSN :
- 14230097 and 10182438
- Volume :
- 151
- Database :
- OpenAIRE
- Journal :
- International Archives of Allergy and Immunology
- Accession number :
- edsair.doi.dedup.....5a36fc283a1fc7739b18e925b2751af2