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Isolation and Screening of Extracellular PGPR from the Rhizosphere of Tomato Plants after Long-Term Reduced Tillage and Cover Crops
- Source :
- Plants, Volume 9, Issue 5, Plants, Vol 9, Iss 668, p 668 (2020)
- Publication Year :
- 2020
-
Abstract
- Plant growth promoting rhizobacteria provide an innovative solution to address challenges in sustainable agro-ecosystems, improving plant growth as well as acting as agents of biocontrol. In this study autochthonous bacteria were isolated from the rhizosphere of processing tomato plants (Solanum lycopersicum L.) cultivated with conservation agriculture practices (i.e., reduced tillage and cover crops), and evaluated for both growth-promoting activities (PGPAs), and antagonistic potential against the phytopathogenic pest Sclerotinia sclerotiorum. Considering the several activities of PGPR, we decided to structure the screening with a hierarchic approach, starting from testing the capability of fixing nitrogen. The obtained bacteria were processed through the molecular typing technique rep-PCR (Repetitive Extragenic Palindromic) in order to discriminate microbial strains with the same profiles, and identified via 16S rDNA sequencing. Thirty-eight selected isolates were screened in vitro for different activities related to plant nutrition and plant growth regulation as well as for antifungal traits. Isolated bacteria were found to exhibit different efficiencies in indoleacetic acid production and siderophore production, phosphate solubilization and biocontrol activity against the widespread soil-borne plant pathogen S. sclerotiorum. All the 38 bacterial isolates showed at least one property tested. With a view to detect the suitable candidates to be developed as biofertilizers, the selected isolates were ranked by their potential ability to function as PGPR. Thus, consortium of native PGPR bacteria inoculants may represent a suitable solution to address the challenges in sustainable agriculture, to ensure crop yield and quality, lowering the application of chemicals input.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Siderophore
Biofertilizer
Plant Science
Biology
Rhizobacteria
01 natural sciences
Article
03 medical and health sciences
Settore AGR/13 - CHIMICA AGRARIA
IAA production
diazotrophs
Microbial inoculant
Ecology, Evolution, Behavior and Systematics
Rhizosphere
Ecology
business.industry
siderophores
Sclerotinia sclerotiorum
fungi
Botany
food and beverages
biology.organism_classification
Biotechnology
biostimulants
030104 developmental biology
conservation agriculture
QK1-989
Settore AGR/16 - MICROBIOLOGIA AGRARIA
Nitrogen fixation
phosphate solubilizers
Solanum
business
Settore AGR/02 - AGRONOMIA E COLTIVAZIONI ERBACEE
010606 plant biology & botany
Subjects
Details
- ISSN :
- 22237747
- Volume :
- 9
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Plants (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....5a285b4c351f16d4fc685705d49ea38b