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Photochemical inactivation with amotosalen and long-wavelength ultraviolet light of Plasmodium and Babesia in platelet and plasma components
- Source :
- Transfusion, Transfusion, Wiley, 2008, 48 (8), pp.1676-1684. ⟨10.1111/j.1537-2995.2007.01762.x⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- BACKGROUND: Transfusion-transmitted cases of malaria and babesiosis have been well documented. Current efforts to screen out contaminated blood products result in component wastage due to the lack of specific detection methods while donor deferral does not always guarantee safe blood products. This study evaluated the efficacy of a photochemical treatment (PCT) method with amotosalen and long-wavelength ultraviolet light (UVA) to inactivate these agents in red blood cells (RBCs) contaminating platelet (PLT) and plasma components. STUDY DESIGN AND METHODS:Plasmodium falciparum– and Babesia microti–contaminated RBCs seeded into PLT and plasma components were treated with 150 µmol per L amotosalen and 3 J per cm2 UVA. The viability of both pathogens before and after treatment was measured with infectivity assays. Treatment with 150 µmol per L amotosalen and 1 J per cm2 UVA was used to assess the robustness of the PCT system. RESULTS: No viable B. microti was detected in PLTs or plasma after treatment with 150 mol per L amotosalen and 3 J per cm2 UVA, demonstrating a mean inactivation of greater than 5.3 log in PLTs and greater than 5.3 log in plasma. After the same treatment, viable P. falciparum was either absent or below the limit of quantification in three of four replicate experiments both in PLTs and in plasma demonstrating a mean inactivation of at least 6.0 log in PLTs and at least 6.9 log in plasma. Reducing UVA dose to 1 J per cm2 did not significantly affect the level of inactivation. CONCLUSION:P. falciparum and B. microti were highly sensitive to inactivation by PCT. Pathogen inactivation approaches could reduce the risk of transfusion-transmitted parasitic infections and avoid unnecessary donor exclusions.
- Subjects :
- Blood Platelets
Amotosalen
Erythrocytes
Photochemistry
Ultraviolet Rays
[SDV]Life Sciences [q-bio]
Plasmodium falciparum
Immunology
Blood Component Transfusion
Blood Donors
030204 cardiovascular system & hematology
Babesia microti
Plasmodium
Mice
Plasma
03 medical and health sciences
0302 clinical medicine
Babesiosis
Furocoumarins
parasitic diseases
medicine
Ultraviolet light
Animals
Humans
Immunology and Allergy
[CHIM]Chemical Sciences
Platelet
030212 general & internal medicine
Malaria, Falciparum
ComputingMilieux_MISCELLANEOUS
Infectivity
biology
Hematology
medicine.disease
biology.organism_classification
3. Good health
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Babesia
Blood Component Removal
Subjects
Details
- Language :
- English
- ISSN :
- 00411132 and 15372995
- Database :
- OpenAIRE
- Journal :
- Transfusion, Transfusion, Wiley, 2008, 48 (8), pp.1676-1684. ⟨10.1111/j.1537-2995.2007.01762.x⟩
- Accession number :
- edsair.doi.dedup.....5a1b0d4f0e6385312393df990e933d76