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AtaT Improves the Stability of Pore-Forming Protein EspB by Acetylating Lysine 206 to Enhance Strain Virulence
- Source :
- Frontiers in Microbiology, Vol 12 (2021), Frontiers in Microbiology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- A novel type II toxin of toxin–antitoxin systems (TAs), Gcn5-related N-acetyltransferase (GNAT) family, was reported recently. GNAT toxins are mainly present in pathogenic species, but studies of their involvement in pathogenicity are rare. This study discovered that the GANT toxin AtaT in enterohemorrhagic Escherichia coli (EHEC) can significantly enhance strain pathogenicity. First, we detected the virulence of ΔataT and ΔataR in cell and animal models. In the absence of ataT, strains showed a lower adhesion number, and host cells presented weaker attaching and effacing lesions, inflammatory response, and pathological injury. Next, we screened the acetylation substrate of AtaT to understand the underlying mechanism. Results showed that E. coli pore-forming protein EspB, which acts as a translocon in type III secretion system (T3SS) in strains, can be acetylated specifically by AtaT. The acetylation of K206 in EspB increases protein stability and maintains the efficiency of effectors translocating into host cells to cause close adhesion and tissue damage.
- Subjects :
- Microbiology (medical)
lcsh:QR1-502
Virulence
medicine.disease_cause
Microbiology
Pore forming protein
lcsh:Microbiology
Type three secretion system
03 medical and health sciences
enterohemorrhagic Escherichia coli
medicine
EspB
Escherichia coli
AtaT
Original Research
030304 developmental biology
0303 health sciences
030306 microbiology
Effector
Toxin
Chemistry
Gcn5-related N-acetyltransferase
Translocon
virulence
Acetylation
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....5a15c470b6deab897a6f88d94c17daf0
- Full Text :
- https://doi.org/10.3389/fmicb.2021.627141