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Silver-amplified fluorescence immunoassay via aggregation-induced emission for detection of disease biomarker
- Source :
- Talanta. 225
- Publication Year :
- 2020
-
Abstract
- Development of simple, robust, and reliable detection strategy of disease biomarkers holds tremendous promise for early clinical diagnosis and prognosis of diseases. In this work, through combining a silver nanoparticle (AgNP) linked immunoassay and aggregation induced emission (AIE)-based fluorogenic Ag+ probe, we developed a silver-amplified fluorescence immunoassay for the detection of disease biomarkers. This method overcame the intrinsic limitations of enzymes as the dissolution of AgNPs generated numerous Ag+, which could switch on the fluorogenic Ag+ probe driven by tetrazolate-Ag+ complexation. As a proof of concept, our method could be used for determining α-fetoprotein (AFP) with a linear relationship in concentrations ranging from 0.1 ng mL−1 to 5 μg mL−1 and a low limit of detection of 42 pg mL−1. Our method was successfully confirmed for the detection of AFP in real serum samples from hepatocellular carcinoma (HCC) patients, demonstrating the great potential for clinical diagnosis.
- Subjects :
- Carcinoma, Hepatocellular
Silver
Metal Nanoparticles
02 engineering and technology
01 natural sciences
Silver nanoparticle
Analytical Chemistry
medicine
Disease biomarker
Humans
Aggregation-induced emission
Detection limit
Immunoassay
Chromatography
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Liver Neoplasms
Fluorescence immunoassay
021001 nanoscience & nanotechnology
0104 chemical sciences
Clinical diagnosis
alpha-Fetoproteins
0210 nano-technology
Biosensor
Subjects
Details
- ISSN :
- 18733573
- Volume :
- 225
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....5a143745d7abc20f7de5445d543e14a1