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Alterations in cardiac structure and function in a modified rat model of myocardial hypertrophy
- Source :
- Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 34(5)
- Publication Year :
- 2014
-
Abstract
- This study was aimed to establish a stable animal model of left ventricular hypertrophy (LVH) to provide theoretical and experimental basis for understanding the development of LVH. The abdominal aorta of male Wistar rats (80-100 g) was constricted to a diameter of 0.55 mm between the branches of the celiac and anterior mesenteric arteries. Echocardiography using a linear phased array probe was performed as well as pathological examination and plasma B-type natriuretic peptide (BNP) measurement at 3, 4 and 6 weeks after abdominal aortic constriction (AAC). The results showed that the acute mortality rate (within 24 h) of this modified rat model was 8%. Animals who underwent AAC demonstrated significantly increased interventricular septal (IVS), LV posterior wall (LVPWd), LV mass index (LVMI), cross-sectional area (CSA) of myocytes, and perivascular fibrosis; the ejection fraction (EF), fractional shortening (FS), and cardiac output (CO) were consistently lower at each time point after AAC. Notably, differences in these parameters between AAC group and sham group were significant by 3 weeks and reached peaks at 4th week. Following AAC, the plasma BNP was gradually elevated compared with the sham group at 3rd and 6th week. It was concluded that this modified AAC model can develop LVH, both stably and safely, by week four post-surgery; echocardiography is able to assess changes in chamber dimensions and systolic properties accurately in rats with LVH.
- Subjects :
- Male
medicine.medical_specialty
Cardiac output
Time Factors
Biomedical Engineering
Enzyme-Linked Immunosorbent Assay
Constriction, Pathologic
Left ventricular hypertrophy
Biochemistry
Muscle hypertrophy
Constriction
Biomaterials
Internal medicine
medicine.artery
Natriuretic Peptide, Brain
Genetics
Medicine
Animals
cardiovascular diseases
Aorta, Abdominal
Rats, Wistar
Mesenteric arteries
Earth-Surface Processes
Aorta
Ejection fraction
business.industry
Myocardium
Abdominal aorta
Heart
medicine.disease
Disease Models, Animal
medicine.anatomical_structure
Echocardiography
Cardiology
Hypertrophy, Left Ventricular
business
Subjects
Details
- ISSN :
- 16720733
- Volume :
- 34
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban
- Accession number :
- edsair.doi.dedup.....59f7ef10f886ea80e2c9868482883f0f