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Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2017
-
Abstract
- The yeast microtubule polymerase Stu2’s C-terminal domain is a 15-nm parallel, homodimeric coiled coil with two spatially distinct conserved regions. Determinants in these conserved regions optimally position Stu2 on the mitotic spindle to drive proper spindle structure and dynamics.<br />XMAP215/Dis1 family proteins are potent microtubule polymerases, critical for mitotic spindle structure and dynamics. While microtubule polymerase activity is driven by an N-terminal tumor overexpressed gene (TOG) domain array, proper cellular localization is a requisite for full activity and is mediated by a C-terminal domain. Structural insight into the C-terminal domain’s architecture and localization mechanism remain outstanding. We present the crystal structure of the Saccharomyces cerevisiae Stu2 C-terminal domain, revealing a 15-nm parallel homodimeric coiled coil. The parallel architecture of the coiled coil has mechanistic implications for the arrangement of the homodimer’s N-terminal TOG domains during microtubule polymerization. The coiled coil has two spatially distinct conserved regions: CRI and CRII. Mutations in CRI and CRII perturb the distribution and localization of Stu2 along the mitotic spindle and yield defects in spindle morphology including increased frequencies of mispositioned and fragmented spindles. Collectively, these data highlight roles for the Stu2 dimerization domain as a scaffold for factor binding that optimally positions Stu2 on the mitotic spindle to promote proper spindle structure and dynamics.
- Subjects :
- 0301 basic medicine
Saccharomyces cerevisiae Proteins
Protein domain
Saccharomyces cerevisiae
Spindle Apparatus
Microtubules
Microtubule polymerization
03 medical and health sciences
Protein Domains
Microtubule
Tubulin
Kinetochores
Molecular Biology
Cellular localization
Cytoskeleton
Coiled coil
biology
Kinetochore
Cell Biology
Articles
3. Good health
Cell biology
Spindle apparatus
030104 developmental biology
biology.protein
Protein Structural Elements
Microtubule-Associated Proteins
Protein Binding
Subjects
Details
- ISSN :
- 19394586
- Volume :
- 29
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular biology of the cell
- Accession number :
- edsair.doi.dedup.....59d9a2406ffba2fbefc427c5eae43170