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Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis

Authors :
In-Hyuk Ha
Junseon Lee
Hyunseong Kim
Jin Young Hong
Wan-Jin Jeon
Source :
PLoS ONE, PLoS ONE, Vol 16, Iss 5, p e0251464 (2021)
Publication Year :
2021
Publisher :
Public Library of Science (PLoS), 2021.

Abstract

Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. We assessed the degree of damage and locomotor function of an LSS model in Sprague-Dawley rats using silicone blocks of varying hardness (70, 80, and 90 kPa) implanted at the L4 level. In sham rats, the spinal cord remained intact; in LSS rats, the spinal cord was increasingly compressed by the mechanical pressure of the silicone blocks as hardness increased. Inflammatory cells were not evident in sham rats, but numerous inflammatory cells were observed around the implanted silicone block in LSS rats. CD68+ cell quantification revealed increases in the inflammatory response in a hardness-dependent manner in LSS rats. Compared with those in sham rats, proinflammatory cytokine levels were significantly elevated in a hardness-dependent manner, and locomotor function was significantly decreased, in LSS rats. Overall, this study showed that hardness could be used as an index to control the severity of nerve injury induced by silicone implants.

Details

ISSN :
19326203
Volume :
16
Database :
OpenAIRE
Journal :
PLOS ONE
Accession number :
edsair.doi.dedup.....59d853829f3daa2addf174d8fb09dc89
Full Text :
https://doi.org/10.1371/journal.pone.0251464