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Effective photoacoustic absorption spectrum for collagen-based tissue imaging
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2020
- Publisher :
- SPIE-Intl Soc Optical Eng, 2020.
-
Abstract
- Significance: Collagen is a basic component of many tissues such as tendons, muscles, and skin, and its imaging helps diagnose and monitor treatments in a variety of fields, including orthopedics. However, due to the overlapping peaks of the absorption spectrum with water in the short-wave infrared region (SWIR), it is difficult to select an optimal wavelength and obtain the photoacoustic (PA) image for collagen-based tissues. Therefore, an additional approach to selecting the proper wavelength is needed. Aim: The aim of this study is to derive an effective PA absorption spectrum of collagen to select the optimal wavelength for high-sensitive PA imaging (PAI). Approach: We measure the absorption spectrum by acquiring the PA signal from various collagen-based samples. To derive an effective PA absorption spectrum in the SWIR band, the following two parameters should be considered: (1) the laser excitation for generating the PA signal and (2) the absorption spectrum for water in the SWIR band. This molecular intrinsic property suggests the optimal wavelength for high-sensitive PAI of collagen-based samples. Results: PA absorption spectral peaks of collagen were found at wavelengths of 1200, 1550, and 1700 nm. Thereby, the PA signal increased by up to five times compared with the wavelength commonly used in collagen PAI. We applied a pulsed fiber laser with a center wavelength of 1560 nm, and the three-dimensional PA image of a collagen patch was obtained. Conclusions: The effective PA absorption spectrum contributes to the improvement of the PA image sensitivity by presenting the optimal wavelength of the target samples.
- Subjects :
- Paper
collagen
Materials science
Absorption spectroscopy
Infrared
Biomedical Engineering
01 natural sciences
Signal
Imaging
law.invention
Photoacoustic Techniques
010309 optics
Biomaterials
fiber laser
Imaging, Three-Dimensional
law
Fiber laser
0103 physical sciences
Absorption (electromagnetic radiation)
Photoacoustic spectroscopy
absorption spectrum
Lasers
Spectrum Analysis
short-wave infrared
Laser
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Wavelength
photoacoustic imaging
Biomedical engineering
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....59d257a52619c3b7d3f745accff2fb86