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mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27
- Source :
- Oncotarget
- Publication Year :
- 2015
-
Abstract
- // Zhuoying Feng 1, * , Luping Zhang 1, * , Junchen Zhou 1 , Shuai Zhou 1 , Li li 1 , Xuyan Guo 1 , Guoying Feng 1 , Ze Ma 1 , Wenhua Huang 2 , Fei Huang 1 1 Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, Laishan District, Shandong Province, 264003,China 2 Institute of Clinical Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China * These authors contributed to this work equally and should be considered as co-first authors Correspondence to: Fei Huang, email: hfei22518@163.com Wenhua Huang, email: huangwenhua2009@139.com Keywords: gliomas, mir-218-2, CDC27, proliferation, invasion Received: December 28, 2015 Accepted: November 02, 2016 Published: December 10, 2016 ABSTRACT Glioma has become a significant global health problem with substantial morbidity and mortality, underscoring the importance of elucidating its underlying molecular mechanisms. Recent studies have identified mir-218 as an anti-oncogene; however, the specific functions of mir-218-1 and mir-218-2 remain unknown, especially the latter. The objective of this study was to further investigate the role of mir-218-2 in glioma. Our results indicated that mir-218-2 is highly overexpressed in glioma. Furthermore, we showed that mir-218-2 is positively correlated with the growth, invasion, migration, and drug susceptibility (to β-lapachone) of glioma cells. In vitro , the overexpression of mir-218-2 promoted glioma cell proliferation, invasion, and migration. In addition, the overexpression of mir-218-2 in vivo was found to increase glioma tumor growth. Accordingly, the inhibition of mir-218-2 resulted in the opposite effects. Cell division cycle 27 (CDC27), the downstream target of mir-218-2, is involved in the regulation of glioma cells. Our results indicate that the overexpression of CDC27 counteracted the function of mir-218-2 in glioma cells. These novel findings provide new insight in the application of mir-218-2 as a potential glioma treatment.
- Subjects :
- 0301 basic medicine
Male
Time Factors
mir-218-2
proliferation
Mice, Nude
Antineoplastic Agents
Drug resistance
Glioma cell
Biology
Clinical anatomy
Transfection
Gene Expression Regulation, Enzymologic
Cell division cycle
03 medical and health sciences
0302 clinical medicine
Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome
CDC27
In vivo
Cell Movement
Glioma
Cell Line, Tumor
medicine
Animals
Humans
Tumor growth
Neoplasm Invasiveness
Cell Proliferation
Mice, Inbred BALB C
Brain Neoplasms
medicine.disease
invasion
Xenograft Model Antitumor Assays
nervous system diseases
Tumor Burden
Gene Expression Regulation, Neoplastic
gliomas
MicroRNAs
030104 developmental biology
Oncology
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Immunology
Cancer research
Glioblastoma
Naphthoquinones
Signal Transduction
Research Paper
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....59c237c6d9b406bc3e4b6b2a27e96f3c