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An exercise in molecular epidemiology: Human rhinovirus prevalence and genetics
- Source :
- Biochemistry and Molecular Biology Education
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Human rhinovirus (HRV) is one of the most common human respiratory pathogens and is responsible for the majority of upper respiratory illnesses. Recently, a phylogeny was constructed from all known American Type Culture Collection (ATCC) HRV sequences. From this study, three HRV classifications (HRVA, HRVB, and HRVC) were determined and techniques for classifying new isolates of HRV were reported. The genetic change of this virus in specific populations over time is of great interest to understand the evolution and epidemiology of viruses. To facilitate the collections of HRV sequences over a number of years, a virology experiment was designed in which students test nasal lavage samples to look for HRV infection. Students will learn a variety of techniques including RNA isolation, cDNA synthesis, qPCR, and agarose gel electrophoresis as well as bioinformatic skills though examination of sequences from the HRV‐field isolates. Furthermore, students can look at symptom data from subjects to investigate correlations between symptom severity and factors such as stress and sleep patterns. Such information can be used to examine hypotheses regarding HRV mutation, symptom severity and epidemiology. BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION Vol. 39, No. 6, pp. 457–458, 2011
- Subjects :
- medicine.medical_specialty
Biomedical Research
Rhinovirus
Sequence analysis
Genomics
virus
Biology
medicine.disease_cause
Biochemistry
Virus
Evolution, Molecular
Laboratory Exercise
stomatognathic system
Virology
Epidemiology
genomics
Medical Laboratory Science
Prevalence
otorhinolaryngologic diseases
medicine
Humans
Respiratory Tract Infections
Molecular Biology
Phylogeny
Genetics
Molecular Epidemiology
Picornaviridae Infections
Molecular epidemiology
Respiratory tract infections
Incidence (epidemiology)
virus diseases
Sequence Analysis, DNA
qPCR
RNA
Education, Medical, Undergraduate
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 14708175
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Biochemistry and Molecular Biology Education
- Accession number :
- edsair.doi.dedup.....59c0967e4d3370321ffb1db96a4d7911