Back to Search
Start Over
Application of quantitative gene expression analysis for pertussis vaccine safety control
- Source :
- Vaccine. 26:4686-4696
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Although vaccines are routinely used to prevent infectious diseases, little is known about the comprehensive influences caused by vaccines. In this study, we showed, using comprehensive gene expression analysis, that pertussis vaccine affected many genes in multiple organs of vaccine-treated animals. In particular, lung was revealed to be the most suitable target to evaluate pertussis vaccine toxicity. The 13 genes identified from the analysis of vaccine-treated lung at day 1 showed a clear dendrogram corresponding to pertussis vaccine toxicity. Furthermore, quantitative analysis of these genes revealed a positive correlation between their respective expression levels and the degree of toxic effects observed in samples that had been treated with various doses of reference pertussis vaccines. The quantification of this 13 gene-set is an indicator of the vaccine toxicity-related reaction.
- Subjects :
- Male
Microarray
Positive correlation
Gene expression
medicine
Animals
Rats, Wistar
Lung
Gene
Whooping cough
Oligonucleotide Array Sequence Analysis
Pertussis Vaccine
General Veterinary
General Immunology and Microbiology
business.industry
Gene Expression Profiling
Body Weight
Public Health, Environmental and Occupational Health
Safety control
medicine.disease
Virology
Rats
Up-Regulation
Infectious Diseases
Immunology
Toxicity
Molecular Medicine
Pertussis vaccine
business
medicine.drug
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....599ec8333da1693ab1dc372883281981