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Downregulation of Phospholipase C δ3 by cAMP and Calcium
- Source :
- Biochemical and Biophysical Research Communications. 286:274-280
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- Four different isoforms of mammalian phospholipase C delta (PLCdelta) have been described. PLCdelta1, the best-understood isoform, is activated by an atypical GTP-binding protein. It has been suggested that it is a calcium signal amplifier. However, very less is known about other subtypes, including PLCdelta3. Therefore, in the present study, we examined the expression of PLCdelta3 in different human tissues. Moreover, the cellular underlying regulation for PLCdelta3 was studied in different cell lines. Our study showed that the mRNA and protein levels differed significantly among human tissues. The human PLCdelta3 gene was composed of 15 exons and 1 putative cAMP response element in the 5'-end promoter region. PLCdelta3 mRNA expression was downregulated by cAMP and calcium in both the human normal embryonic lung tissue diploid WI38 cell line and the glioblastoma/astrocytoma U373 cell line. However, mRNA expression showed no impact by PKC activators or inhibitors. This study shows the human PLCdelta3 expression pattern and is the first report that PLCdelta3 gene expression is downregulation by cAMP and calcium.
- Subjects :
- Gene isoform
Response element
Biophysics
Down-Regulation
chemistry.chemical_element
Calcium
Response Elements
Biochemistry
Cell Line
Downregulation and upregulation
Gene expression
Cyclic AMP
Tumor Cells, Cultured
Humans
Tissue Distribution
Calcium Signaling
RNA, Messenger
Promoter Regions, Genetic
Molecular Biology
Calcimycin
Protein Kinase C
Protein kinase C
Ionophores
biology
Phospholipase C
Cell Biology
Molecular biology
Cell biology
Isoenzymes
Bucladesine
chemistry
Type C Phospholipases
biology.protein
CREB1
Phospholipase C delta
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....598f572e743abb9a77a1d5d0ad0dfe0a
- Full Text :
- https://doi.org/10.1006/bbrc.2001.5371