How to predict product yield in kinetically controlled enzymatic peptide synthesis

The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters α and β for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH2 from Mal-L-Phe-L-AlaOMe and L-LeuNH2. The experimental results demonstrate that this theory adequately describes the real process. © 1992 John Wiley & Sons, Inc.