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Measurement of Angstrom to Nanometer Molecular Distances with 19F Nuclear Spins by EPR/ENDOR Spectroscopy
- Source :
- Angewandte Chemie (International Ed. in English), Angewandte Chemie International Edition
- Publication Year :
- 2019
- Publisher :
- John Wiley and Sons Inc., 2019.
-
Abstract
- Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio‐macromolecules using 19F nuclear spins and nitroxide radicals in combination with high‐frequency (94 GHz/3.4 T) electron–nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19F label enables to access distances up to about 1.5 nm with an accuracy of 0.1–1 Å. The experiment is not limited by the size of the bio‐macromolecule. Performance is illustrated on synthesized fluorinated model compounds as well as spin‐labelled RNA duplexes. The results demonstrate that our simple but strategic spin‐labelling procedure combined with state‐of‐the‐art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in the solution state.<br />What did the NO say to the 19 F? Dipolar couplings between nitroxide spin labels and 19F nuclear spins report inter‐spin distances in the range of ≲15 Å at sub‐angstrom accuracy. These couplings are measured with sub‐nanomole spin sensitivity by high frequency (94 GHz) electron–nuclear double resonance (ENDOR) and can be employed to obtain detailed structural insights into biomolecules.
- Subjects :
- Models, Molecular
Nitroxide mediated radical polymerization
Materials science
Structure Determination
Gyromagnetic ratio
chemistry.chemical_element
010402 general chemistry
01 natural sciences
Molecular physics
Catalysis
law.invention
law
RNA
fluorine
high field ENDOR
spin labelling
structural biology
Humans
Spectroscopy
Electron paramagnetic resonance
Research Articles
Spins
010405 organic chemistry
Electron Spin Resonance Spectroscopy
Resonance
General Chemistry
0104 chemical sciences
Structural biology
chemistry
Fluorine
Spin Labels
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15213773 and 14337851
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie (International Ed. in English)
- Accession number :
- edsair.doi.dedup.....5968177a6b662267fde17ba2a39be8cf