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Differential effect of IL-1β and TNFα on the production of IL-6, IL-8 and PGE2 in fibroblast-like synoviocytes and THP-1 macrophages
- Source :
- Rheumatology international. 30(8)
- Publication Year :
- 2009
-
Abstract
- Inflammation in the joint of rheumatoid arthritis is a complex immune reaction facilitated by various factors, such as cytokines, cells and hypoxia. Thus, we evaluated their relative capacity to produce proinflammatory mediators in response to IL-1beta, TNF-alpha or IL-17 under hypoxia or normoxia in fibroblast-like synoviocytes (FLSs) and macrophages. The level of IL-6 expression was strongly increased in both FLSs and THP-1 macrophages in response to IL-1beta and TNF-alpha, but the level by TNF-alpha was less than that by IL-1beta. In contrast, the expression of IL-8 in both cell types was strongly stimulated by both IL-1beta and TNF-alpha. In FLSs, PGE(2) production increased only in response to IL-1beta; and no effect was observed in THP-1 cells and TNF-alpha-stimulated FLSs. In addition, the production by IL-17 was extremely low when compared with those induced by IL-1beta or TNF-alpha in FLSs and THP-1 cells. Hypoxia (2% O(2)) decreased IL-1beta-stimulated production of PGE(2), even though it increased the expression of mRNA and protein of COX-2. These results suggest that IL-1beta and TNF-alpha differentially regulate gene expression in FLSs and macrophages under hypoxia or normoxia.
- Subjects :
- Fibroblast-like synoviocyte
Immunology
Interleukin-1beta
Inflammation
Dinoprostone
Proinflammatory cytokine
Rheumatology
Cell Line, Tumor
medicine
Immunology and Allergy
Humans
THP1 cell line
Interleukin 8
Interleukin 6
Fibroblast
Cells, Cultured
biology
business.industry
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophages
Interleukin-8
Synovial Membrane
Fibroblasts
Molecular biology
Up-Regulation
medicine.anatomical_structure
Gene Expression Regulation
Cell culture
biology.protein
medicine.symptom
business
Subjects
Details
- ISSN :
- 1437160X
- Volume :
- 30
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Rheumatology international
- Accession number :
- edsair.doi.dedup.....59618d4f60d700c293b7004920460a53