Permethrin resistance associated with inherited genes in a near‐isogenic line of Musca domestica

Background Permethrin has been used frequently in the control of Musca domestica worldwide including Pakistan, with reports of resistance development in different field strains. A near-isogenic line of M. domestica with permethrin resistance (Perm-R) was constructed to elucidate mode of inheritance and cross-resistance analyses. Results Toxicity responses of reciprocal progenies F1 and F1' showed no significant difference in median lethal dose (LD50 ) values, suggesting that permethrin resistance was inherited in an autosomal fashion with neither sex linkage nor maternal effects in the Perm-R strain of M. domestica. Degree of dominance was 0.6 and 0.7 for F1 and F1' progenies, respectively, which suggests that permethrin resistance was expressed as an incompletely dominant trait. Chi-square analyses for self-bred (F2), and backcross progenies (BC1, BC2, BC3 and BC4) revealed significant differences between the observed and expected mortality, indicating the possibility of multiple genes responsible for permethrin resistance. Moreover, the Perm-R strain did not show cross-resistance to propoxur, chlorpyrifos, profenofos or spinetoram. Conclusion Permethrin resistance in the Perm-R strain of M. domestica was inherited as autosomal, incompletely dominant and governed by more than one gene. Lack of cross-resistance between permethrin and propoxur, chlorpyrifos, profenofos or spinetoram provides an opportunity for rotational use of these insecticides in the control of M. domestica. These data could help to manage M. domestica and the problem of permethrin resistance.