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1,25(OH)2D3 inhibits in vitro and in vivo intracellular growth of apicomplexan parasite Toxoplasma gondii
- Source :
- Journal of Steroid Biochemistry and Molecular Biology, Journal of Steroid Biochemistry and Molecular Biology, Elsevier, 2007, 103 (3-5), pp.811-4. ⟨10.1016/j.jsbmb.2006.12.058⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- The hormonal form of vitamin D, 1,25-dyhydroxyvitamin D3 (1,25(OH)2D3), is implicated in a wide range of functions other than its classical role in calcium and phosphorous homeostasis. When Toxoplasma gondii -infected BALB/c mice were treated with 1,25(OH)2D3, they succumb to death sooner than their counterparts. But they showed less parasite burden in tissues which was further supported by mild pathological lesions. As an effort to understand the physiological mechanism for the above observation an in vitro study was performed. Fewer parasites were observed when 1,25(OH)2D3 pre-treated murine intestinal epithelial cells were challenged with parasites. Moreover, the observed inhibition was dose-dependent and had a maximum effect with 10 −7 M of 1,25(OH)2D3. However, no observable difference was observed, when pre-incubated parasites were added to cells suggesting that the observed inhibition was a result of an effect from 1,25(OH)2D3 on Toxoplasma intracellular growth. Our data support the notion that 1,25(OH)2D3 may inhibit intra cellular T. gondii parasite proliferation in vivo and in vitro.
- Subjects :
- Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
MESH: Mice, Inbred BALB C
chemistry.chemical_element
Calcium
Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Endocrinology
Calcitriol
In vivo
Parasite hosting
Animals
MESH: Animals
Molecular Biology
MESH: Mice
030304 developmental biology
0303 health sciences
Mice, Inbred BALB C
biology
Cell growth
MESH: Toxoplasma
Toxoplasma gondii
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
biology.organism_classification
Molecular biology
In vitro
3. Good health
Cell biology
chemistry
MESH: Calcitriol
030220 oncology & carcinogenesis
MESH: Toxoplasmosis
Molecular Medicine
Female
Toxoplasma
MESH: Female
Intracellular
Homeostasis
Toxoplasmosis
Subjects
Details
- Language :
- English
- ISSN :
- 09600760
- Database :
- OpenAIRE
- Journal :
- Journal of Steroid Biochemistry and Molecular Biology, Journal of Steroid Biochemistry and Molecular Biology, Elsevier, 2007, 103 (3-5), pp.811-4. ⟨10.1016/j.jsbmb.2006.12.058⟩
- Accession number :
- edsair.doi.dedup.....592a8002928184e4bf6344195d2bff11
- Full Text :
- https://doi.org/10.1016/j.jsbmb.2006.12.058⟩