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Functional diversification of a basic helix–loop–helix protein due to alternative transcription during generation of amphidiploidy in tobacco plants
- Source :
- Biochemical Journal. 403:493-499
- Publication Year :
- 2007
- Publisher :
- Portland Press Ltd., 2007.
-
Abstract
- A plastid-resident basic helix–loop–helix protein, previously identified in Nicotiana tabacum and designated as NtWIN4 (N. tabacum wound-induced clone 4), has been converted from a nuclear transcription repressor into a plastid-resident regulatory factor through replacement of the DNA-binding domain with a plastid transit sequence during evolution. N. tabacum is a natural amphidiploid plant derived from Nicotiana tomentosiformis and Nicotiana sylvestris and immunoblot staining using anti-NtWIN4 antibodies identified two protein species, a 26 kDa form and a 17 kDa form, in N. sylvestris, whereas only the 17 kDa form was found in N. tabacum. The 26 kDa protein is produced when translation starts from the first AUG codon of the mRNA and is predominantly localized in the cytoplasm and nucleus, whereas the 17 kDa protein is derived from a 24 kDa precursor protein, synthesized from the second AUG codon, and localizes only to plastids. Subsequent analyses revealed that the lengths of the mRNAs vary in the two plant species. One major form lacks the first AUG, while minor populations possess variable 5′-untranslated regions prior to the first AUG codon. Translation of the two types produces the 24 kDa and 26 kDa proteins respectively. In vitro translation assays indicated that initiation frequency from the first AUG codon is higher in mRNAs from N. sylvestris than from N. tabacum. In contrast, initiation from the second AUG codon was found to be equally efficient in mRNAs from both species. These results suggest that both mRNA populations and translation efficiency changed during the amphidiploidization responsible for generation of N. tabacum. This scheme could reflect a molecular mechanism of protein evolution in plants.
- Subjects :
- Untranslated region
Plastid localization
Transcription, Genetic
Nicotiana tabacum
Molecular Sequence Data
Commentary Article
Repressor
Biology
Biochemistry
Evolution, Molecular
Polyploidy
Start codon
Tobacco
Amino Acid Sequence
Plastids
RNA, Messenger
Molecular Biology
Helix-Turn-Helix Motifs
Plant Proteins
Genetics
Messenger RNA
fungi
food and beverages
Cell Biology
biology.organism_classification
Molecular biology
Repressor Proteins
Nicotiana tomentosiformis
Nicotiana sylvestris
Sequence Alignment
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 403
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....59111ceb5a7acd79c8263534d1bd71be
- Full Text :
- https://doi.org/10.1042/bj20070011