Back to Search
Start Over
Simultaneous Saccharification and Co-fermentation with a Thermotolerant Saccharomyces cerevisiae to Produce Ethanol from Sugarcane Bagasse under High Temperature Conditions
- Publication Year :
- 2021
- Publisher :
- NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI, 2021.
-
Abstract
- Lignocellulosic ethanol production at high temperature offers advantages such as the decrease of contamination risk and cooling cost. Recombinant xylose-fermenting Saccharomyces cerevisiae has been considered a promising strain for ethanol production from lignocellulose for its high inhibitor tolerance and superior capability to ferment glucose and xylose into ethanol. To improve the ethanolic fermentation by xylose at high temperature, the strain YY5A was subjected to the ethyl methanesulfonate (EMS) mutagenesis. A mutant strain T5 was selected from the EMS-treated cultures to produce ethanol. However, the xylose uptake by T5 was severely inhibited by the high ethanol concentration during the co-fermentation in defined YPDX medium at 40 °C. In this study, the simultaneous saccharification and co-fermentation (SSCF) and the separate hydrolysis and co-fermentation (SHCF) processes of sugarcane bagasse were assessed to solve this problem. The xylose utilization by T5 was remarkably improved using the SSCF process compared to the SHCF process. For the SHCF and SSCF processes, 48% and 99% of the xylose in the hydrolysate was consumed at 40 °C, respectively. The ethanol yield was enhanced by the SSCF process. The ethanol production can reach to 36.0 g/L using this process under high-temperature conditions.
- Subjects :
- STOVER
Co-fermentation
Technology
Environmental Engineering
Materials Science, Paper & Wood
Materials Science
XYLOSE-ISOMERASE
Bioengineering
Sugarcane bagasse
Saccharomyces cerevisiae
Xylose
Hydrolysate
chemistry.chemical_compound
Hydrolysis
Ethanol fuel
Food science
Waste Management and Disposal
Inhibitor tolerance
Ethanol
Science & Technology
BIOETHANOL PRODUCTION
STRAINS
YEASTS
Simultaneous saccharification and co-fermentation
INDUSTRIAL
chemistry
Lignocellulosic ethanol
Mutagenesis
KLUYVEROMYCES-MARXIANUS
ACID
Fermentation
Bagasse
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....58fa1b3115ede09f26c7ddea62018d5a