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Identification of Cancer Stem Cell Molecular Markers and Effects of hsa-miR-21-3p on Stemness in Esophageal Squamous Cell Carcinoma
- Source :
- Cancers, Cancers, Vol 11, Iss 4, p 518 (2019), Volume 11, Issue 4
- Publication Year :
- 2019
-
Abstract
- Cancer stem cells (CSCs) are closely related to tumor resistance and tumor recurrence in esophageal squamous cell carcinoma (ESCC). The lack of specific biomarkers to identify and isolate CSCs has led to the slow progression of research on CSCs in ESCC. Here, we established a method to identify and isolate CSCs in ESCC using fluorescence-activated cell sorting with combined surface biomarkers including CD71, CD271, and CD338. CD71&minus<br />/CD271+/CD338+ subpopulation cells possessed more stem cell properties in proliferation, self-renewal, differentiation, metastasis, drug resistance, and tumorigenesis. We further explored possible roles that microRNAs played in stem cells. Using microarrays, we identified that has-miR-21-3p was highly expressed in positive sorted cells, and further functional and Luciferase reporter assays verified that has-miR-21-3p promoted proliferation and anti-apoptosis by regulating TRAF4. We further analyzed the relationship between hsa-miR-21-3p and ESCC in 137 patients with ESCC. Statistical analysis showed that up-regulation of hsa-miR-21-3p was associated with a high risk of ESCC. Collectively, we identified surface biomarkers of stem cells in esophageal squamous cell carcinoma, and discovered thathsa-miR-21-3p may be involved in stemness maintenance by regulating TRAF4.
- Subjects :
- 0301 basic medicine
Cancer Research
cancer stem cell
TRAF4
Transferrin receptor
Biology
medicine.disease_cause
lcsh:RC254-282
Article
Metastasis
03 medical and health sciences
0302 clinical medicine
Cancer stem cell
microRNA
medicine
neoplasms
Hsa-miR-21-3p
Cell sorting
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
digestive system diseases
esophageal squamous cell carcinoma
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Stem cell
Carcinogenesis
Subjects
Details
- ISSN :
- 20726694
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cancers
- Accession number :
- edsair.doi.dedup.....58ec1d4448f82b35528aec20f35195f2