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Phenotypic and Transcriptomic Lymphocytes Changes in Allograft Recipients After Intravenous Immunoglobulin Therapy in Kidney Transplant Recipients
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- High dose intravenous immunoglobulin (IVIG) are widely used after kidney transplantation and its biological effect on T and B cell phenotype in the context of maintenance immunosuppression was not documented yet. We designed a monocentric prospective cohort study of kidney allograft recipients with anti-HLA donor specific antibodies (DSA) without acute rejection on screening biopsies treated with prophylactic high-dose IVIG (2 g/kg) monthly for 2 months. Any previous treatment with Rituximab was an exclusion criterion. We performed an extensive analysis of phenotypic and transcriptomic T and B lymphocytes changes and serum cytokines after treatment (day 60). Twelve kidney transplant recipients who completed at least two courses of high-dose IVIG (2 g/kg) were included in a median time of 45 (12–132) months after transplant. Anti-HLA DSA characteristics were similar before and after treatment. At D60, PBMC population distribution was similar to the day before the first infusion. CD8+ CD45RA+ T cells and naïve B-cells (Bm2+) decreased (P = 0.03 and P = 0.012, respectively) whereas Bm1 (mature B-cells) increased (P = 0.004). RORγt serum mRNA transcription factor and CD3 serum mRNA increased 60 days after IVIG (P = 0.02 for both). Among the 25 cytokines tested, only IL-18 serum concentration significantly decreased at D60 (P = 0.03). In conclusion, high dose IVIG induced limited B cell and T cell phenotype modifications that could lead to anti-HLA DSA decrease. However, no clinical effect has been isolated and the real benefit of prophylactic use of IVIG after kidney transplantation merits to be questioned.
- Subjects :
- Graft Rejection
Male
0301 basic medicine
medicine.medical_treatment
immunomodulation
Gastroenterology
high-dose intravenous immunoglobulin
0302 clinical medicine
Intravenous Immunoglobulin Therapy
HLA Antigens
Immunology and Allergy
Medicine
Lymphocytes
Prospective Studies
Kidney transplantation
Original Research
education.field_of_study
Graft Survival
Immunoglobulins, Intravenous
Immunosuppression
Middle Aged
Allografts
Tissue Donors
Phenotype
medicine.anatomical_structure
Female
Rituximab
donor specific antibodies
medicine.drug
lcsh:Immunologic diseases. Allergy
Adult
medicine.medical_specialty
T cell
Population
Immunology
kidney transplantation
03 medical and health sciences
Internal medicine
lymphocytes phenotype
Humans
Transplantation, Homologous
education
B cell
Aged
business.industry
Immunization, Passive
medicine.disease
Transplant Recipients
030104 developmental biology
Immunoglobulin G
lcsh:RC581-607
Transcriptome
business
CD8
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....58e0ae7675e36c3dcd37663acda6aa4a