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Macrobrachium rosenbergii nodavirus infection in M. rosenbergii (de Man) with white tail disease cultured in Taiwan
- Source :
- Journal of fish diseases. 31(6)
- Publication Year :
- 2008
-
Abstract
- White tail disease (WTD) is a serious problem in Macrobrachium rosenbergii hatcheries and nursery ponds in Asia. The causative agents have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus. This is the first report demonstrating MrNV virus in M. rosenbergii displaying WTD signs in Taiwan by reverse transcriptase-polymerase chain reaction (RT-PCR). Amplified fragments of 850 and 425 bp for RNA-1 and RNA-2 of MrNV, respectively, were obtained by RT-PCR. RT-PCR products of about 850 and 1121 bp for RNA-1 and RNA-2 of MrNV were also obtained using different primer pairs. The amplicons were individually cloned into pGEM-T vector and sequenced. Using this recombinant plasmid of MrNV RNA-2 as DNA template, the non-radioactive DNA probes were prepared by PCR amplification with DIG-11-dUTP. The probes were used to successfully detect MrNV infection in the striated muscle tissues of WTD-diseased prawns using in situ hybridization. The 1121 bp genomic fragment of RNA-2 of MrNV consisted of a unique open reading frame with 1116 nucleotides, and it encoded a structural protein with 371 amino acids. The nucleotide sequence of the partial genome of MrNV RNA-2 revealed a 97% identity with an Indian isolate. A phylogenetic tree constructed using the nucleotide sequence of the viral capsid gene from insect and fish nodaviruses revealed that the MrNV Taiwan isolate could be interpreted as a new genus within the family Nodaviridae. However, its position showed more affinity with Alphanodavirus than with Betanodavirus. The study confirmed the presence of MrNV infection in freshwater prawns cultured in Taiwan suffering from WTD.
- Subjects :
- biology
Base Sequence
Macrobrachium rosenbergii
Veterinary (miscellaneous)
Hybridization probe
Molecular Sequence Data
Nucleic acid sequence
Betanodavirus
Taiwan
Aquatic Science
Amplicon
biology.organism_classification
Virology
Muscle, Striated
law.invention
Viral Proteins
Capsid
law
Animals
Nodaviridae
Amino Acid Sequence
Primer (molecular biology)
Palaemonidae
Polymerase chain reaction
Phylogeny
Subjects
Details
- ISSN :
- 01407775
- Volume :
- 31
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of fish diseases
- Accession number :
- edsair.doi.dedup.....58dfa34032d2d7b2a31ae4fd9b988e26