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Caco-2 cell line as a model to evaluate mucoprotective proprieties
- Publication Year :
- 2012
-
Abstract
- Physical protection of mucosa surface and reduction of inflammatory processes are currently considered the main strategies in the treatment and prevention of mucosal diseases. However, the majority of models used to verify the activity of new mucoprotective agents are based on limiting instrumental assessment or the sacrifice of experimental animals. In this study, for the first time, some in vitro experimental methods using Caco-2 cell line are proposed as predicting in vivo behaviour and action of mucoprotective agents. To this purpose, hyaluronic acid and natural polysaccharides for their bioadhesive activity, hydrocortisone and natural polyphenols as anti-inflammatory agents have been chosen. The obtained results demonstrated that the techniques (Con A/o-pd assay and Franz cell system) of mucoadhesive evaluation on Caco-2 cells are useful to compare the activity of each experimental sample and to assess the adhesion time to the mucosal cell surface. Moreover, the reduction of intercellular adhesion molecule-1 (ICAM-1) expression in Caco-2 cells can be considered directly correlated to the mucosal anti-inflammatory effect induced by the hydrocortisone and natural polyphenols. In conclusion, the study supported the use of Caco-2 cell as a model to compare and investigate the effect of different active substances on the mucosa and its diseases.
- Subjects :
- Time Factors
Hydrocortisone
Bioadhesive
Cell
Anti-Inflammatory Agents
Pharmaceutical Science
Pharmacology
Animal Testing Alternatives
Binding, Competitive
chemistry.chemical_compound
In vivo
Hyaluronic acid
medicine
Concanavalin A
Humans
Hyaluronic Acid
Intestinal Mucosa
ICAM-1
Chemistry
Adhesiveness
Polyphenols
Intercellular Adhesion Molecule-1
In vitro
medicine.anatomical_structure
Caco-2
Cell culture
Cytoprotection
Immunology
Caco-2 Cells
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....58d7359b2ca0863fe9749258d20b3428