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Type I and type II interferons delay human neutrophil apoptosis via activation of STAT3 and up-regulation of cellular inhibitor of apoptosis 2
- Source :
- Journal of leukocyte biology. 78(1)
- Publication Year :
- 2005
-
Abstract
- We have recently demonstrated that granulocyte-colony stimulating factor (G-CSF) delays human neutrophil apoptosis via up-regulation of cellular inhibitor of apoptosis 2 (cIAP2), which is dependent on activation of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3). Here, we show that type I and type II interferons (IFNs), which bind to the distinct receptors, exert the antiapoptotic effect on human neutrophils through the similar mechanism. IFN-α (type I IFN) and IFN-γ (type II IFN), like G-CSF, delayed human neutrophil apoptosis through the protein synthesis-dependent mechanism. Stimulation of neutrophils with IFN-α or IFN-γ resulted in tyrosine phosphorylation of STAT1 and STAT3 but not phosphorylation of STAT5, Akt, extracellular signal-regulated kinase, and p38 mitogen-activated protein kinase. IFN-α and IFN-γ induced the expression of transcripts of cIAP2 and suppressor of cytokine signaling 1 and 3, but not cIAP1, Mcl-1, and A1. IFN-α- and IFN-γ-induced up-regulation of cIAP2 mRNA and protein, phosphorylation of STAT3, and antiapoptotic effect were inhibited significantly by pretreatment of cells with AG490, a specific inhibitor of JAK2. These findings suggest that cIAP2 expression is up-regulated by IFN-α and IFN-γ through, at least in part, activation of the JAK2-STAT3 pathway, and increased expression of the cIAP2 protein may contribute to an IFN-α- and IFN-γ-mediated antiapoptotic effect on human neutrophils.
- Subjects :
- Adult
STAT3 Transcription Factor
Neutrophils
p38 mitogen-activated protein kinases
Immunology
Apoptosis
Inhibitor of apoptosis
chemistry.chemical_compound
Interferon-gamma
Proto-Oncogene Proteins
Granulocyte Colony-Stimulating Factor
Immunology and Allergy
Humans
STAT1
Enzyme Inhibitors
Phosphorylation
STAT3
Protein kinase A
Protein kinase B
Janus kinase 2
biology
Interferon-alpha
Proteins
Tyrosine phosphorylation
Cell Biology
Janus Kinase 2
Protein-Tyrosine Kinases
Tyrphostins
Cell biology
Up-Regulation
DNA-Binding Proteins
STAT1 Transcription Factor
chemistry
biology.protein
Trans-Activators
Subjects
Details
- ISSN :
- 07415400
- Volume :
- 78
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of leukocyte biology
- Accession number :
- edsair.doi.dedup.....58c80d0ec60d3e3ac923f2a20f960291