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Cytosine deaminase gene as a potential tool for the genetic therapy of colorectal cancer
- Source :
- Journal of surgical oncology. 61(1)
- Publication Year :
- 1996
-
Abstract
- The bacterial enzyme cytosine deaminase (CD) catalyzes the conversion of 5-fluorocytosine (5-FC) to the lethal 5-fluorouracil (5-FU) and so provides a useful system for selective killing of gene-modified mammalian tumor cells. Cloning of the CD gene from Escherichia coli and expression in human tumor cell lines enabled these cells to convert 3H-labeled 5-FC into 3H-5-FU. Two CD-expressing human tumor cell lines (adenocarcinoma cell line KM12 and glioblastoma cell line T1115) became 200-fold more sensitive to 5-FC than the nonexpressing parental cell lines. At least 90% of the cells are killed within 7 days. CD-expressing cells are able to kill nonexpressing cells when grown in the same culture flask (bystander effect). The CD gene may be used as a suicide system for in situ chemotherapy or as a safety mechanism abrogating the expression of other genes. © 1996 Wiley-Liss, Inc.
- Subjects :
- Colorectal cancer
Genetic enhancement
R Factors
Genetic Vectors
Molecular Sequence Data
Flucytosine
Nucleoside Deaminases
Biology
medicine.disease_cause
Transfection
Polymerase Chain Reaction
Cytosine Deaminase
Bystander effect
medicine
Escherichia coli
Tumor Cells, Cultured
Humans
Cloning, Molecular
Gene
Cloning
Base Sequence
Cell Death
Cytosine deaminase
General Medicine
Genetic Therapy
medicine.disease
Oncology
Cell culture
Immunology
Cancer research
Interleukin-2
Surgery
Fluorouracil
Colorectal Neoplasms
Glioblastoma
Subjects
Details
- ISSN :
- 00224790
- Volume :
- 61
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of surgical oncology
- Accession number :
- edsair.doi.dedup.....58bb6c2a778e7494f046f240d4c0b132