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A novel transgenic Cre allele to label mouse cardiac conduction system
- Source :
- Dev Biol
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The cardiac conduction system is a network of heterogeneous cell population that initiates and propagates electric excitations in the myocardium. Purkinje fibers, a network of specialized myocardial cells, comprise the distal end of the conduction system in the ventricles. The developmental origins of Purkinje fibers and their roles during cardiac physiology and arrhythmia have been reported. However, it is not clear if they play a role during ischemic injury and heart regeneration. Here we introduce a novel tamoxifen-inducible Cre allele that specifically labels a broad range of components in the cardiac conduction system while excludes other cardiac cell types and vital organs. Using this new allele, we investigated the cellular and molecular response of Purkinje fibers to myocardial injury. In a neonatal mouse myocardial infarction model, we observed significant increase in Purkinje cell number in regenerating myocardium. RNA-Seq analysis using laser-captured Purkinje fibers showed a unique transcriptomic response to myocardial infarction. Our finds suggest a novel role of cardiac Purkinje fibers in heart injury.
- Subjects :
- Heart Injury
Purkinje fibers
Heart Ventricles
Population
Purkinje cell
Myocardial Infarction
Mice, Transgenic
Biology
Article
Purkinje Fibers
Mice
Heart Conduction System
medicine
Animals
Regeneration
Ventricular Function
Cell Lineage
Myocytes, Cardiac
RNA-Seq
Myocardial infarction
education
Molecular Biology
Alleles
education.field_of_study
Integrases
Myocardium
Regeneration (biology)
Cell Biology
medicine.disease
Cardiovascular physiology
Cell biology
Tamoxifen
medicine.anatomical_structure
Animals, Newborn
cardiovascular system
Electrical conduction system of the heart
Transcriptome
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 478
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....587eb858c887364388c746183d32491a
- Full Text :
- https://doi.org/10.1016/j.ydbio.2021.07.005