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The Protective Effect of Metformin against Oxandrolone-Induced Infertility in Male Rats
- Source :
- Current Pharmaceutical Design. 28:324-330
- Publication Year :
- 2022
- Publisher :
- Bentham Science Publishers Ltd., 2022.
-
Abstract
- Background:Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus.Aim:This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats.Methods:Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR.Results:Oxandrolone administration induced male infertility by significantly reducing relative weights of testes by 48%, sperm count by 82%, and serum testosterone levels by 96% (ANOVA, P value < 0.05). In addition, histological examination determined that oxandrolone caused spermatogenic arrest which was associated with 2-fold downregulation of testicular CYP11A1 gene expression. However, co-administration of metformin with oxandrolone significantly ameliorated toxicological alterations induced by oxandrolone exposure (ANOVA, P value < 0.05).Conclusion:Metformin administration protected against oxandrolone-induced infertility in male rats. Further clinical studies are needed to confirm the protective effect of metformin against oxandrolone-induced infertility among athletes.
- Subjects :
- Male
Infertility
medicine.medical_specialty
Male infertility
Oxandrolone
Diabetes mellitus
Internal medicine
Testis
Drug Discovery
medicine
Animals
Humans
Testosterone
Infertility, Male
Pharmacology
business.industry
medicine.disease
Sperm
Metformin
Rats
Endocrinology
Analysis of variance
business
medicine.drug
Subjects
Details
- ISSN :
- 13816128
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Current Pharmaceutical Design
- Accession number :
- edsair.doi.dedup.....587d83fb11d3101e3d0bbf5c8c47f1cb