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The Murine Misty Mutation: Phenotypic Effects on Melanocytes, Platelets and Brown Fat
- Source :
- Genetics. 148:381-390
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- Although the recessive murine mutation misty (m) is well known, its phenotype has never been reported beyond brief descriptions of a dilution of coat color and white spotting of the belly and extremities, suggesting a developmental mutation. A report in abstract has also suggested effects on white fat and body weight. Here, we report effects of the homozygous misty mutation on an unusual combination of three cell types: melanocytes, platelets, and brown fat. Brown fat appeared to be completely absent from all expected locations in neonatal m/m mice. A prolonged bleeding time was observed; platelet count and platelet serotonin and ATP levels were normal, but the level of ADP in m/m platelets was low. Primary cultures and immortal lines of melanocytes from m/m mice showed several abnormalities. There was a marked deficiency in net proliferation, suggesting that the color dilution and spotting in vivo may result from reduced numbers of melanocytes and their precursors. m/m melanocytes were also hyperdendritic in morphology, overproduced melanin, and had deficient responses to the cAMP agonists cholera toxin and melanocyte-stimulating hormone, which normally promote melanin production. The misty gene product may be involved in adenine nucleotide metabolism or signaling.
- Subjects :
- Blood Platelets
Bleeding Time
White adipose tissue
Biology
medicine.disease_cause
Gene product
Melanin
Mice
Adenosine Triphosphate
Adipose Tissue, Brown
Adenine nucleotide
Genetics
medicine
Animals
Platelet
Hair Color
Mice, Inbred C3H
Mutation
Pigmentation
Cholera toxin
Phenylthiourea
Phenotype
Mice, Inbred C57BL
Genes
Melanocytes
Cell Division
Research Article
Subjects
Details
- ISSN :
- 19432631
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Genetics
- Accession number :
- edsair.doi.dedup.....58791dc5c57d9318a5fa965be844019e