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Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
- Source :
- SEDICI (UNLP), Universidad Nacional de La Plata, instacron:UNLP, CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2019
-
Abstract
- Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in in vivo inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further in vivo and in vitro studies to understand GAL's modulatory effects are warranted.<br />Facultad de Ciencias Veterinarias<br />Instituto de Estudios Inmunológicos y Fisiopatológicos
- Subjects :
- Male
Medicina
medicine.medical_treatment
Ischemia
Pharmacology
Mannans
03 medical and health sciences
Mice
0302 clinical medicine
Downregulation and upregulation
Digestive system surgical procedures
medicine.artery
medicine
Animals
Humans
Superior mesenteric artery
Intestinal Mucosa
Interleukin 6
Receptor
biology
Chemistry
Galactose
purl.org/becyt/ford/3.1 [https]
medicine.disease
Small intestine
Intestine
Reperfusion injury
Mesenteric ischemia
Disease Models, Animal
medicine.anatomical_structure
Cytokine
Jejunum
030220 oncology & carcinogenesis
Reperfusion Injury
biology.protein
030211 gastroenterology & hepatology
Surgery
purl.org/becyt/ford/3 [https]
Subjects
Details
- ISSN :
- 10958673
- Volume :
- 249
- Database :
- OpenAIRE
- Journal :
- The Journal of surgical research
- Accession number :
- edsair.doi.dedup.....586b169bf733fbbb7e45ed11f811a720