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Molecular Characterization of Enterotoxigenic Escherichia coli StrainsIsolated from Diarrheal Patients in Korea during 2003-2011
- Source :
- PLOS ONE(9): 5, PLoS ONE, PLoS ONE, Vol 9, Iss 5, p e96896 (2014)
- Publication Year :
- 2014
-
Abstract
- Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of infectious diarrhea in developing countries. In order to characterize the molecular features of human ETEC isolates from Korea, we investigated the profiles of enterotoxin and colonization factor (CF) genes by polymerase chain reaction (PCR) and performed multilocus sequence typing (MLST) with a total of 291 ETEC strains. The specimens comprised 258 domestic strains isolated from patients who had diarrhea and were from widely separated geographic regions in Korea and 33 inflow strains isolated from travelers visiting other Asian countries. Heat-stable toxin (STh)-possessing ETEC strains were more frequent than heat-labile toxin (LT)-possessing ETEC strains in the domestic isolates, while the detection rates of both enterotoxin genes were similar in the inflow isolates. The profile of CF genes of domestic isolates was similar to that of inflow isolates and the major CF types of the strains were CS3-CS21-CS1/PCF071 and CS2-CS3-CS21. Most of these 2 CF types were detected in ETEC strains that possess both lt and sth genes. The major MLSTST types of domestic isolates were ST171 and ST955. Moreover, the 2 major CF types were usually found concomitantly with the 2 major MLST STs, ST171 and ST955. In conclusion, our genotyping results may provide useful information for guiding the development of geographically specific vaccines against human ETEC isolates.
- Subjects :
- Health Screening
lcsh:Medicine
Enterotoxin
Pathogenesis
medicine.disease_cause
Pathology and Laboratory Medicine
law.invention
law
Enterotoxigenic Escherichia coli
Genotype
Medicine and Health Sciences
Gastrointestinal Infections
Public and Occupational Health
lcsh:Science
Polymerase chain reaction
Escherichia Coli
Multidisciplinary
Escherichia coli Proteins
Bacterial Pathogens
Diarrhea
Infectious Diseases
Medical Microbiology
medicine.symptom
Bacterial and Foodborne Illness
Environmental Health
Research Article
Microbial Sensitivity Tests
Gastroenterology and Hepatology
Biology
Microbiology
Antibiotic resistance
Republic of Korea
medicine
Humans
Genotyping
Gram Negative Bacteria
Microbial Pathogens
Population Biology
lcsh:R
Biology and Life Sciences
Bacteriology
Virology
Health Care
Emerging Infectious Diseases
Multilocus sequence typing
lcsh:Q
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLOS ONE(9): 5, PLoS ONE, PLoS ONE, Vol 9, Iss 5, p e96896 (2014)
- Accession number :
- edsair.doi.dedup.....58663321469f54ddb4f3405bb67e5f47