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Genome-Wide Gene Expression Analysis Reveals Unique Genes Signatures of Epithelial Reorganization in Primary Airway Epithelium Induced by Type-I, -II and -III Interferons
- Source :
- Biosensors 12:929 (2022), Biosensors; Volume 12; Issue 11; Pages: 929
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Biosensors such as toll-like receptors (TLR) induce the expression of interferons (IFNs) after viral infection that are critical to the first step in cell-intrinsic host defense mechanisms. Their differential influence on epithelial integrity genes, however, remains elusive. A genome-wide gene expression biosensor chip for gene expression sensing was used to examine the effects of type-I, -II, and -III IFN stimulation on the epithelial expression profiles of primary organotypic 3D air-liquid interface airway cultures. All types of IFNs induced similar interferon-stimulated genes (ISGs): OAS1, OAS2, and IFIT2. However, they differentially induced transcription factors, epithelial modulators, and pro-inflammatory genes. Type-I IFN-induced genes were associated with cell–cell adhesion and tight junctions, while type-III IFNs promoted genes important for transepithelial transport. In contrast, type-II IFN stimulated proliferation-triggering genes associated and enhanced pro-inflammatory mediator secretion. In conclusion, with our microarray system, we provide evidence that the three IFN types exceed their antiviral ISG-response by inducing distinct remodeling processes, thereby likely strengthening the epithelial airway barrier by enhancing cross-cell-integrity (I), transepithelial transport (III) and finally reconstruction through proliferation (II).
- Subjects :
- Clinical Biochemistry
Biomedical Engineering
Gene Expression
General Medicine
Antiviral Agents
Epithelium
Analytical Chemistry
ddc
Article
microarray
gene expression analysis
airway epithelial
type-I
II and -III Interferons
epithelial integrity
remodeling
Interferon Type I
ii And -iii Interferons
Airway Epithelial
Epithelial Integrity
Gene Expression Analysis
Microarray
Remodeling
Type-i
Instrumentation
Engineering (miscellaneous)
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Biosensors 12:929 (2022), Biosensors; Volume 12; Issue 11; Pages: 929
- Accession number :
- edsair.doi.dedup.....5866214b26ea735f636b72edb40eebe3