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Impact of Long-Term HFD Intake on the Peripheral and Central IGF System in Male and Female Mice
- Source :
- Metabolites, Biblos-e Archivo. Repositorio Institucional de la UAM, instname, Volume 10, Issue 11, Metabolites, Vol 10, Iss 462, p 462 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- The insulin-like growth factor (IGF) system is responsible for growth, but also affects metabolism and brain function throughout life. New IGF family members (i.e., pappalysins and stanniocalcins) control the availability/activity of IGFs and are implicated in growth. However, how diet and obesity modify this system has been poorly studied. We explored how intake of a high-fat diet (HFD) or commercial control diet (CCD) affects the IGF system in the circulation, visceral adipose tissue (VAT) and hypothalamus. Male and female C57/BL6J mice received HFD (60% fat, 5.1 kcal/g), CCD (10% fat, 3.7 kcal/g) or chow (3.1 % fat, 3.4 kcal/g) for 8 weeks. After 7 weeks of HFD intake, males had decreased glucose tolerance (p &lt<br />0.01) and at sacrifice increased plasma insulin (p &lt<br />0.05) and leptin (p &lt<br />0.01). Circulating free IGF1 (p &lt<br />0.001), total IGF1 (p &lt<br />0.001), IGF2 (p &lt<br />0.05) and IGFBP3 (p &lt<br />0.01) were higher after HFD in both sexes, with CCD increasing IGFBP2 in males (p &lt<br />0.001). In VAT, HFD reduced mRNA levels of IGF2 (p &lt<br />0.05), PAPP-A (p &lt<br />0.001) and stanniocalcin (STC)-1 (p &lt<br />0.001) in males. HFD increased hypothalamic IGF1 (p &lt<br />0.01), IGF2 (p &lt<br />0.05) and IGFBP5 (p &lt<br />0.01) mRNA levels, with these changes more apparent in females. Our results show that diet-induced changes in the IGF system are tissue-, sex- and diet-dependent.
- Subjects :
- 0301 basic medicine
sex differences
medicine.medical_specialty
obesity
Medicina
Endocrinology, Diabetes and Metabolism
lcsh:QR1-502
IGFBP3
Diet and obesity
Neuropeptide
Adipose tissue
030209 endocrinology & metabolism
Biochemistry
lcsh:Microbiology
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
Sex differences
medicine
Obesity
Molecular Biology
business.industry
Leptin
Neuropeptides
digestive, oral, and skin physiology
IGF1
IGF2
neuropeptides
medicine.disease
030104 developmental biology
Endocrinology
High-fat diet
high-fat diet
Hypothalamus
IGFBP2
Decreased glucose tolerance
business
Subjects
Details
- Language :
- English
- ISSN :
- 22181989
- Volume :
- 10
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Metabolites
- Accession number :
- edsair.doi.dedup.....5864459b0f5121a312eabd983ba49468