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Detection of Leishmania (Viannia) braziliensis in bats from Espírito Santo, Brazil (2018–2019)
- Source :
- Parasitology Research. 120:3857-3863
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Studying the occurrence of Leishmania in bats would help toward clarifying the role of these animals in epidemiological cycles. Therefore, the objective of this study was to detect Leishmania spp. in bats from the Brazilian State of Espírito Santo. In total, 105 bats from 11 species which had been collected from 17 municipalities of Espírito Santo were obtained from the IDAF Rabies Diagnostic Laboratory between December 2018 and June 2019. DNA was extracted from the bone marrow, skin, and spleen samples and analysed using conventional PCR, with primers specific for the kinetoplastic DNA minicircle of the parasitic species Leishmania (Viannnia) braziliensis, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) amazonensis. To identify the Leishmania species, the positive PCR products were analysed using PCR-RFLP with the HaeIII enzyme and 8% polyacrylamide gel electrophoresis. Positivity for L. (V.) braziliensis was observed in five (4.76%) bats: namely, one male Eumops glaucinus from Vitória (in a skin sample), one male Artibeus lituratus from Vila Velha and one female Eumops glaucinus from Vitória (both in bone marrow samples), and one male A. lituratus from Vitória and one male Molossus rufus from Guarapari (both in the spleen samples). To our best knowledge, this is the first report of the detection of Leishmania in bats from Espírito Santo as well as of Leishmania DNA in the bone marrow of bats.
- Subjects :
- Male
Veterinary medicine
Espirito santo
Leishmania braziliensis
law.invention
HaeIII
law
Chiroptera
parasitic diseases
medicine
Leishmania (Viannia) braziliensis
Animals
Eumops glaucinus
Leishmania infantum
Artibeus
Polymerase chain reaction
General Veterinary
biology
DNA, Kinetoplast
General Medicine
Leishmania
biology.organism_classification
medicine.disease
Infectious Diseases
Insect Science
Female
Parasitology
Rabies
Brazil
medicine.drug
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....5860c6632519602b8bc24b161303d2eb