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Hsa_circ_0030586 promotes epithelial–mesenchymal transition in prostate cancer via PI3K-AKT signaling
- Source :
- Bioengineered, Vol 12, Iss 2, Pp 11089-11107 (2021), Bioengineered, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Circular RNAs (CircRNAs) gain importance as regulatory molecules in prostate cancer (PCa), but molecular mechanism of most circRNAs in pathogenesis of PCa remains to be studied. This study aimed to explore the role of hsa_circ_0030586 in PCa. Gene Expression Omnibus database (GSE77661) was used to screen out candidate circRNAs. Quantitative real-time PCR was used to verify the relative expressions of circRNAs, miRNAs, and genes in PCa cells. A CCK-8 assay was used to evaluate the cells’ proliferation. Transwell and wound healing assay were used to determine the cells’ migration and invasion. Western blotting and immunohistochemistry were used to detect the protein expression of PI3K/AKT signaling proteins and epithelial–mesenchymal transition (EMT) markers. Furthermore, a nude mice tumorigenesis experiment in vivo was conducted to determine the function of hsa_circ_0030586 on PCa. Our results showed that hsa_circ_0030586 is significantly upregulated in PCa cells (p
- Subjects :
- Male
Epithelial-Mesenchymal Transition
Carcinogenesis
Mice, Nude
circular rna
Bioengineering
epithelial–mesenchymal transition
medicine.disease_cause
Applied Microbiology and Biotechnology
Phosphatidylinositol 3-Kinases
Downregulation and upregulation
Cell Movement
Cell Line, Tumor
microRNA
medicine
Animals
Humans
rna sequencing
Neoplasm Invasiveness
RNA, Messenger
Epithelial–mesenchymal transition
Protein kinase B
PI3K/AKT/mTOR pathway
Cell Proliferation
Mice, Inbred BALB C
Base Sequence
Chemistry
Cell growth
Gene Expression Profiling
Prostatic Neoplasms
Reproducibility of Results
RNA, Circular
General Medicine
Transfection
has_circ_0030586
prostate cancer
Up-Regulation
Gene Expression Regulation, Neoplastic
pi3k-akt
Cancer research
Proto-Oncogene Proteins c-akt
TP248.13-248.65
Research Article
Research Paper
Signal Transduction
Biotechnology
Subjects
Details
- ISSN :
- 21655987 and 21655979
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....584f3608f5cfcc31e4a8a1d7c7479862