Back to Search
Start Over
Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway
- Source :
- Cell Death and Disease, Vol 9, Iss 4, Pp 1-11 (2018), Cell Death & Disease
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group, 2018.
-
Abstract
- The overexpression of stomatin-like protein-2 (SLP-2) is commonly observed in non-small cell lung cancer (NSCLC) cells. In the present study, we transfected a number of NSCLC cells with an SLP-2 shRNA-expressing vector (AdSLP2i) and examined its possible effects on cell growth and apoptosis. We found that suppression of SLP-2 expression inhibited cell growth, and that the apoptosis induced by SLP-2 suppression was correlated with decreased survivin protein expression. Moreover, the reduced survivin expression was found to be associated with reduced β-catenin nuclear localization and appeared not to be modulated through the AKT signaling pathway. By using immunoprecipitation and proteomics to analyze protein–protein interactions in A549 cells with SLP-2 overexpression, we found that annexin A2 interacted with SLP-2 and β-catenin directly. Our data further suggested that the knockdown of SLP-2 gene affected the SLP-2/Annexin A2/β-catenin cascade formation, reduced the translocation of cytoplasmic β-catenin into nucleus, and downregulated downstream target genes. The results presented in this study, together with our previous findings, suggest that SLP-2 promotes NSCLC cell proliferation by enhancing survivin expression mediated via β-catenin pathway.
- Subjects :
- Proteomics
0301 basic medicine
Cancer Research
Lung Neoplasms
genetic structures
Survivin
Immunology
Apoptosis
Article
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
Humans
Protein Interaction Maps
RNA, Small Interfering
lcsh:QH573-671
Promoter Regions, Genetic
beta Catenin
Cell Nucleus
A549 cell
Gene knockdown
Cell growth
Chemistry
Akt/PKB signaling pathway
lcsh:Cytology
Membrane Proteins
Blood Proteins
Cell Biology
equipment and supplies
eye diseases
Cell biology
030104 developmental biology
Cell culture
030220 oncology & carcinogenesis
RNA Interference
sense organs
Signal transduction
Peptides
Annexin A2
Protein Binding
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20414889
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cell Death and Disease
- Accession number :
- edsair.doi.dedup.....583fac9f76b848af2b31fa35cf58153d