Back to Search
Start Over
Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target
- Source :
- Nature Communications, Vol 10, Iss 1, Pp 1-7 (2019), Nature Communications
- Publication Year :
- 2019
- Publisher :
- Nature Portfolio, 2019.
-
Abstract
- Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and demonstrate its application to visualizing inflammatory atherosclerotic plaques in vivo. Through a systematic transporter screen using a CRISPR activation library, we identify the orphan transporter Slc18b1/SLC18B1 as the gating target of CDg16.<br />Attempts to image activated macrophages in vivo have been hampered by selectivity and delivery problems. Here the authors develop a small molecule fluorescent probe specific to activated M1 and M2 macrophages, identify the orphan receptor Slc18b1/SLC18B1 as the mechanism of uptake, and use it to image atherosclerosis in mice.
- Subjects :
- 0301 basic medicine
Mice, Knockout, ApoE
Science
Molecular Probe Techniques
General Physics and Astronomy
Inflammation
02 engineering and technology
Gating
Article
General Biochemistry, Genetics and Molecular Biology
Mice
03 medical and health sciences
In vivo
medicine
Animals
Humans
Macrophage
lcsh:Science
Cation Transport Proteins
Multidisciplinary
Chemistry
Transporter
General Chemistry
Macrophage Activation
021001 nanoscience & nanotechnology
Small molecule
Plaque, Atherosclerotic
Cell biology
RAW 264.7 Cells
030104 developmental biology
Molecular Probes
Acridines
lcsh:Q
CRISPR-Cas Systems
medicine.symptom
0210 nano-technology
Molecular probe
HeLa Cells
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....581dfac5f3b193f9ccf3d7fa9e8f6c19