Back to Search
Start Over
Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa)
- Source :
- PLoS ONE, Vol 16, Iss 10, p e0257896 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Introduction Peripheral artery disease (PAD) is a highly morbid condition in which impaired blood flow to the limbs leads to pain and tissue loss. Previously we identified 670 nm electromagnetic energy (R/NIR) to increase nitric oxide levels in cells and tissue. NO elicits relaxation of smooth muscle (SMC) by stimulating potassium efflux and membrane hyperpolarization. The actions of energy on ion channel activity have yet to be explored. Here we hypothesized R/NIR stimulates vasodilation through activation of potassium channels in SMC. Methods Femoral arteries or facial arteries from C57Bl/6 and Slo1-/- mice were isolated, pressurized to 60 mmHg, pre-constricted with U46619, and irradiated twice with energy R/NIR (10 mW/cm2 for 5 min) with a 10 min dark period between irradiations. Single-channel K+ currents were recorded at room temperature from cell-attached and excised inside-out membrane patches of freshly isolated mouse femoral arterial muscle cells using the patch-clamp technique. Results R/NIR stimulated vasodilation requires functional activation of the large conductance potassium channels. There is a voltage dependent outward current in SMC with light stimulation, which is due to increases in the open state probability of channel opening. R/NIR modulation of channel opening is eliminated pharmacologically (paxilline) and genetically (BKca α subunit knockout). There is no direct action of light to modulate channel activity as excised patches did not increase the open state probability of channel opening. Conclusion R/NIR vasodilation requires indirect activation of the BKca channel.
- Subjects :
- Patch-Clamp Techniques
Potassium Channels
Physiology
Potassium
Vasodilation
Smooth Muscle Cells
Vascular Medicine
Biochemistry
Muscle, Smooth, Vascular
Ion Channels
Membrane Potentials
Gene Knockout Techniques
Mice
chemistry.chemical_compound
Single Channel Recording
Spectrum Analysis Techniques
Animal Cells
Medicine and Health Sciences
Myocyte
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
Membrane Electrophysiology
Mice, Knockout
Multidisciplinary
Electromagnetic Radiation
Physics
near-Infrared Spectroscopy
Neurochemistry
Arteries
Potassium channel
Femoral Artery
Electrophysiology
Bioassays and Physiological Analysis
Physical Sciences
Medicine
Neurochemicals
Anatomy
Cellular Types
Signal Transduction
Research Article
Science
Myocytes, Smooth Muscle
Biophysics
Muscle Tissue
Neurophysiology
chemistry.chemical_element
Electric Stimulation Therapy
Infrared Spectroscopy
Nitric Oxide
Research and Analysis Methods
Nitric oxide
Peripheral Arterial Disease
Animals
Paxilline
Muscle Cells
Electrophysiological Techniques
Biology and Life Sciences
Proteins
Conductance
Cell Biology
Membrane hyperpolarization
Femoral Arteries
Electric Stimulation
Biological Tissue
chemistry
Face
Cardiovascular Anatomy
Blood Vessels
Head
Neuroscience
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....581767ec0b9d58b9a2811eafff173079