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Synovial fluid CD34− CD44+ CD90+ mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis
- Source :
- Osteoarthritis and Cartilage. (2):106-109
- Publisher :
- Published by Elsevier Ltd.
-
Abstract
- SummaryTo the best of our knowledge, no reports have directly compared synovial fluid (SF)- and synovial membrane (SM)-derived mesenchymal stem cells (MSCs) from primary knee osteoarthritis patients in terms of MSC proportion, either immediately after isolation or during culture. Any possible correlation between SM- and SF-MSC purity and osteoarthritis severity, also remains unclear. We therefore assessed quantitative and phenotypic differences in MSCs isolated from SF and SM. We also evaluated the correlation between sample MSC purity, and disease severity, in patients with osteoarthritis. The main result of the current study was that the mean SF-MSC proportion at passage 0 was negatively correlated with Kellgren–Lawrence (KL) grade (r = −0.565, P = 0.002). In addition, KL grade was a only significant independent negative predictor of SF-MSC proportion at passage 0 (β = −0.356, P = 0.039). Conclusively, the proportion of SF-MSCs in fresh samples, evaluated at the single cell level, was inversely correlated with osteoarthritis severity.
- Subjects :
- Adult
Male
medicine.medical_specialty
Pathology
CD34
Biomedical Engineering
Antigens, CD34
Osteoarthritis
Gastroenterology
Severity of Illness Index
Immunophenotyping
Rheumatology
Internal medicine
Severity of illness
Synovium
medicine
Synovial fluid
Humans
CD90
Orthopedics and Sports Medicine
Cells, Cultured
Aged
Mesenchymal stem cell
biology
business.industry
CD44
Synovial Membrane
Cell Differentiation
Mesenchymal Stem Cells
Middle Aged
Osteoarthritis, Knee
medicine.disease
medicine.anatomical_structure
Hyaluronan Receptors
biology.protein
Thy-1 Antigens
Female
Synovial membrane
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 10634584
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Osteoarthritis and Cartilage
- Accession number :
- edsair.doi.dedup.....57f64ecbdc3051a4b9a74c0f84ac6595
- Full Text :
- https://doi.org/10.1016/j.joca.2011.11.010