Back to Search
Start Over
The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication
- Source :
- Structure. (11):1905-1917
- Publisher :
- Elsevier Ltd.
-
Abstract
- Summary Centrioles are key microtubule polarity determinants. Centriole duplication is tightly controlled to prevent cells from developing multipolar spindles, a situation that promotes chromosomal instability. A conserved component in the duplication pathway is Plk4, a polo kinase family member that localizes to centrioles in M/G1. To limit centriole duplication, Plk4 levels are controlled through trans -autophosphorylation that primes ubiquitination. In contrast to Plks 1-3, Plk4 possesses a unique central region called the "cryptic polo box." Here, we present the crystal structure of this region at 2.3 A resolution. Surprisingly, the structure reveals two tandem homodimerized polo boxes, PB1-PB2, that form a unique winged architecture. The full PB1-PB2 cassette is required for binding the centriolar protein Asterless as well as robust centriole targeting. Thus, with its C-terminal polo box (PB3), Plk4 has a triple polo box architecture that facilitates oligomerization, targeting, and promotes trans -autophosphorylation, limiting centriole duplication to once per cell cycle.
- Subjects :
- PLK4
Models, Molecular
0303 health sciences
Centriole
Protein Conformation
Autophosphorylation
Polo kinase
Biology
Protein Serine-Threonine Kinases
Crystallography, X-Ray
Article
Cell biology
03 medical and health sciences
0302 clinical medicine
Protein structure
Microtubule
Structural Biology
Gene duplication
Humans
biological phenomena, cell phenomena, and immunity
Multipolar spindles
Molecular Biology
030217 neurology & neurosurgery
030304 developmental biology
Centrioles
Subjects
Details
- Language :
- English
- ISSN :
- 09692126
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Structure
- Accession number :
- edsair.doi.dedup.....57d51141ebf0a7ac399de0d8ca10f253
- Full Text :
- https://doi.org/10.1016/j.str.2012.08.025