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A panel of bioluminescent whole-cell bacterial biosensors for the screening for new antibacterial substances from natural extracts
- Publication Year :
- 2020
-
Abstract
- Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the panel was first evaluated with known antibacterial compounds: ethanol, naphthoquinones (juglone, lawsone, plumbagin) and a flavonoid (quercetin). Natural extracts comprise a vast source of potential new antibacterials for diverse functional purposes. To demonstrate the utilization of the panel for screening of a demanding sample material, round-leaved sundew (Drosera rotundifolia) extracts were used as an example. Differences between field- and laboratory originating sundew extracts could be detected. This demonstrates the efficiency of the developed biosensor panel in the rapid screening of the antibacterial properties of plant extracts. acceptedVersion
- Subjects :
- Microbiology (medical)
chemistry.chemical_classification
0303 health sciences
biology
030306 microbiology
High-throughput screening
Pseudomonas
Flavonoid
116 Chemical sciences
technology, industry, and agriculture
Plumbagin
biology.organism_classification
Microbiology
Lawsone
220 Industrial biotechnology
03 medical and health sciences
chemistry.chemical_compound
Drosera rotundifolia
Biochemistry
chemistry
Molecular Biology
Biosensor
Juglone
030304 developmental biology
219 Environmental biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....57b8a6d3abb41f8da734584f50bfda4a