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Diversity, Antibiotic Resistance, and Biofilm-Forming Ability of Enterobacteria Isolated from Red Meat and Poultry Preparations

Authors :
Patrícia Poeta
Cristina Rodríguez-Melcón
Ana Castaño-Arriba
Rosa Capita
Carlos Alonso-Calleja
Gilberto Igrejas
Source :
Microorganisms, Volume 8, Issue 8, Microorganisms, Vol 8, Iss 1226, p 1226 (2020)
Publication Year :
2020
Publisher :
MDPI, 2020.

Abstract

A total of 44 samples of beef, pork, and poultry preparations were tested. Average counts (log cfu/g) of enterobacteria were 1.99 &plusmn<br />0.99 (beef preparations), 1.96 &plusmn<br />1.44 (pork), 2.09 &plusmn<br />0.92 (chicken), and 2.17 &plusmn<br />1.06 (turkey) (p &gt<br />0.05). Two hundred enterobacterial strains were identified and 13 genera (21 species) were distinguished, including species that are a significant cause of infection. The most common genera were Escherichia (32.5% of strains), Serratia (17.0%), Hafnia (12.5%), and Salmonella (12.0%). Isolates were screened by disc diffusion for susceptibility to 15 antibiotics. A total of 126 strains (63% of the isolates) were multirresistant (having resistance to two or more antibiotics), 46 (23%) were resistant to one antibiotic, and 28 (14%) were sensitive to all antibiotics. The average number of resistances per strain was 2.53 &plusmn<br />2.05. A higher (p &lt<br />0.05) average number of resistances was observed in strains from turkey (3.14 &plusmn<br />2.55) than in strains from beef (2.15 &plusmn<br />1.22), pork (2.16 &plusmn<br />1.39), or chicken (2.44 &plusmn<br />2.22). At least 50% of strains showed resistance or reduced susceptibility to ampicillin, cefotaxime, ceftazidime, or streptomycin, considered to be &ldquo<br />critically important&rdquo<br />antimicrobial agents in human medicine. Seventy-nine strains (39.5%), 60 strains (30.0%), and 46 strains (23.0%) were weak, moderate, and strong biofilm producers (crystal violet assay), respectively. This investigation provides evidence that bacteria from red meat and poultry preparations pose major potential risk to consumers.

Details

Language :
English
ISSN :
20762607
Volume :
8
Issue :
8
Database :
OpenAIRE
Journal :
Microorganisms
Accession number :
edsair.doi.dedup.....57b00cd3bfd19d93cab01838a477338e