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Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test
- Source :
- BioMed Research International, BioMed Research International, Vol 2016 (2016)
- Publication Year :
- 2016
- Publisher :
- Hindawi Publishing Corporation, 2016.
-
Abstract
- Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard. Both WHO HI criteria and IgG ELISA criteria performed strongly (16/16) in determining primary infection. However, to determine secondary infection, the IgG ELISA criteria performed better (72/73) compared to the WHO HI criteria (23/73).
- Subjects :
- 0301 basic medicine
Adult
Male
Hemagglutination Inhibition Tests
Article Subject
Adolescent
Secondary infection
lcsh:Medicine
Enzyme-Linked Immunosorbent Assay
Hemolytic Plaque Technique
Dengue virus
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Immunoglobulin G
Dengue fever
Dengue
03 medical and health sciences
Plaque reduction neutralization test
Neutralization Tests
medicine
Humans
Child
Hemagglutination assay
General Immunology and Microbiology
biology
business.industry
Coinfection
lcsh:R
General Medicine
Gold standard (test)
Dengue Virus
Middle Aged
medicine.disease
Virology
030104 developmental biology
Immunology
biology.protein
Female
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2016
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....57a3961fea3fe8e59041f50b84cb161c