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Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells
- Source :
- Molecules, Volume 24, Issue 24
- Publication Year :
- 2019
-
Abstract
- Inflammation is a common pathogenesis in many diseases. Salvia miltiorrhiza Bunge (Danshen), a traditional Chinese medicine, has been considered to have good anti-inflammatory effects. In the present study, we investigated the anti-inflammatory effect of diethyl blechnic (DB), a novel compound isolated from Danshen, and its possible mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. The results showed that DB can inhibit the LPS-induced pro-inflammatory cytokines release of prostaglandin E2 (PGE2) and mRNA expression of TNF-&alpha<br />IL-6, and IL-1&beta<br />In addition, the results of the flow cytometry assay and the fluorometric intracellular ROS kit assay indicated that DB reduced the generation of ROS in LPS-stimualted RAW264.7 cells. DB reversed the LPS-induced loss of the mitochondrial membrane potential (MMP). Furthermore, DB suppressed the LPS-stimulated increased expression of Toll-like receptor 4 (TLR4), myeloid differential protein-88 (MyD88) and phosphorylation of TAK1, PI3K, and AKT. DB promoted NF-E2-related factor 2 (Nrf2) into the nucleus, increased the expression of heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase [quinone] 1 (NQO1) and reduced the expression of Keap1. In summary, DB may inhibit LPS-induced inflammation, which mainly occurs through TLR4/MyD88 and oxidative stress signaling pathways in RAW264.7 cells.
- Subjects :
- Lipopolysaccharides
Lipopolysaccharide
Anti-Inflammatory Agents
diethyl blechnic
Pharmaceutical Science
Inflammation
Salvia miltiorrhiza
Antioxidants
Article
NF-κB
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
Drug Discovery
medicine
Animals
Physical and Theoretical Chemistry
Protein kinase B
PI3K/AKT/mTOR pathway
030304 developmental biology
Benzofurans
0303 health sciences
Molecular Structure
Macrophages
030302 biochemistry & molecular biology
Organic Chemistry
NF-kappa B
Molecular biology
Toll-Like Receptor 4
Oxidative Stress
RAW 264.7 Cells
chemistry
Chemistry (miscellaneous)
inflammation
Myeloid Differentiation Factor 88
TLR4
Molecular Medicine
Phosphorylation
Cytokines
lipids (amino acids, peptides, and proteins)
medicine.symptom
Signal transduction
Inflammation Mediators
Reactive Oxygen Species
TLR4/MyD88
Signal Transduction
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....57a0151356cab4156d75f6b8ac0252b9