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Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering

Authors :
Seeram Ramakrishna
Balchandar Navaneethan
Shouping Liu
Roger W. Beuerman
Seow Theng Ong
Jayarama Reddy Venugopal
V. Seitz
Rajamani Lakshminarayanan
Navin Kumar Verma
Chetna Dhand
Neeraj Dwivedi
Silvia Marrero Diaz
Erich Wintermantel
Mobashar Hussain Urf Turabe Fazil
Source :
Biomaterials. 104:323-338
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

Bone disorders are the most common cause of severe long term pain and physical disability, and affect millions of people around the world. In the present study, we report bio-inspired preparation of bone-like composite structures by electrospinning of collagen containing catecholamines and Ca(2+). The presence of divalent cation induces simultaneous partial oxidative polymerization of catecholamines and crosslinking of collagen nanofibers, thus producing mats that are mechanically robust and confer photoluminescence properties. Subsequent mineralization of the mats by ammonium carbonate leads to complete oxidative polymerization of catecholamines and precipitation of amorphous CaCO3. The collagen composite scaffolds display outstanding mechanical properties with Young's modulus approaching the limits of cancellous bone. Biological studies demonstrate that human fetal osteoblasts seeded on to the composite scaffolds display enhanced cell adhesion, penetration, proliferation, differentiation and osteogenic expression of osteocalcin, osteopontin and bone matrix protein when compared to pristine collagen or tissue culture plates. Among the two catecholamines, mats containing norepinephrine displayed superior mechanical, photoluminescence and biological properties than mats loaded with dopamine. These smart multifunctional scaffolds could potentially be utilized to repair and regenerate bone defects and injuries.

Details

ISSN :
01429612
Volume :
104
Database :
OpenAIRE
Journal :
Biomaterials
Accession number :
edsair.doi.dedup.....578c90a8b0fb3e084d498a6086c83d7f
Full Text :
https://doi.org/10.1016/j.biomaterials.2016.07.007