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Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering
- Source :
- Biomaterials. 104:323-338
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Bone disorders are the most common cause of severe long term pain and physical disability, and affect millions of people around the world. In the present study, we report bio-inspired preparation of bone-like composite structures by electrospinning of collagen containing catecholamines and Ca(2+). The presence of divalent cation induces simultaneous partial oxidative polymerization of catecholamines and crosslinking of collagen nanofibers, thus producing mats that are mechanically robust and confer photoluminescence properties. Subsequent mineralization of the mats by ammonium carbonate leads to complete oxidative polymerization of catecholamines and precipitation of amorphous CaCO3. The collagen composite scaffolds display outstanding mechanical properties with Young's modulus approaching the limits of cancellous bone. Biological studies demonstrate that human fetal osteoblasts seeded on to the composite scaffolds display enhanced cell adhesion, penetration, proliferation, differentiation and osteogenic expression of osteocalcin, osteopontin and bone matrix protein when compared to pristine collagen or tissue culture plates. Among the two catecholamines, mats containing norepinephrine displayed superior mechanical, photoluminescence and biological properties than mats loaded with dopamine. These smart multifunctional scaffolds could potentially be utilized to repair and regenerate bone defects and injuries.
- Subjects :
- Materials science
Biophysics
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
Calcification, Physiologic
Tissue engineering
Biomimetics
Osteogenesis
Elastic Modulus
Cell Adhesion
medicine
Humans
Osteopontin
Cell adhesion
Cells, Cultured
Cell Proliferation
Bone Development
Osteoblasts
Tissue Engineering
Tissue Scaffolds
biology
Cell Differentiation
Equipment Design
021001 nanoscience & nanotechnology
Electroplating
Electrospinning
0104 chemical sciences
Equipment Failure Analysis
Cross-Linking Reagents
medicine.anatomical_structure
Polymerization
Mechanics of Materials
Nanofiber
Ceramics and Composites
Osteocalcin
biology.protein
Collagen
0210 nano-technology
Cancellous bone
Biomedical engineering
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....578c90a8b0fb3e084d498a6086c83d7f
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2016.07.007